Amino acid derivatives and antihypertensive drugs containing them

ABSTRACT

N-terminal phosphorus dipeptides having antihypertensive properties are disclosed.

This is a continuation-in-part application of U.S. application Ser. No. 458,844 by the same inventors entitled AMINO ACID DERIVATIVES AND ANTIHYPERTENSIVE DRUGS CONTAINING THEM, filed Jan. 18, 1983.

The present invention relates to novel amino acid derivatives and antihypertensive drugs containing them.

The present inventors have succeeded in the synthesis of novel amino acid derivatives represented by the general formula: ##STR1## Moreover, the present inventors have found that these derivatives, having an antihypertensive activity, are useful as antihypertensive drugs and intermediates for their production, and, based on this finding, have completed the present invention.

R₁ and R₂ in the foregoing formula, being the same or different from each other, each individually represents hydroxyl, alkyloxy, aryloxy, aralkyloxy, alkyl, aryl, or aralkyl.

The alkyloxy is a lower alkyloxy having 1˜5 carbon atoms such as methoxy, ethoxy, n-propyloxy, n-butyloxy, and n-pentyloxy.

The aryloxy is the one having 6˜12 carbon atoms such as phenoxy, naphthyloxy, and p-tolyloxy.

The aralkyloxy is the one having 6˜12 carbon atoms such as benzyloxy, phenetyloxy, phenylpropyloxy, and hydroxybenzyloxy.

The alkyl is a lower alkyl having 1˜5 carbon atoms such as methyl, ethyl, n-propyl, n-butyl and n-pentyl.

The aryl is the one having 6˜12 carbon atoms such as phenyl and p-tolyl.

The aralkyl is the one having 6˜12 carbon atoms such as benzyl, phenethyl, and phenylpropyl.

R₃, R₅, R₈, and R₁₁ at least two of which are the same or all of which are different from one another, each individually represents hydrogen atom, or alkyl: for example, the one having 1˜5 carbon atoms such as methyl, ethyl, n-propyl, n-butyl, and n-pentyl. That is to say, all of R₃, R₅, R₈, and R₁₁ may each individually represent hydrogen atom or methyl, or R₃ and R₅ may each individually represent hydrogen atom, and R₈ and R₁₁ may individually represents methyl; all of them may be different from one another: for example, R₃ represents hydrogen atom, R₅ represents methyl, R₈ represents ethyl, and R₁₁ represents isopropyl.

R₄, R₇, and R₁₀ at least two of which are the same or all of which are different from one another, each individually represents hydrogen atom, or substituted or unsubstituted alkyl, aryl, or aralkyl.

The alkyl represents a lower alkyl having 1˜5 carbon atoms such as methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, and n-pentyl.

The aryl represents the one having 6˜12 carbon atoms such as phenyl, p-tolyl, and naphthyl.

The aralkyl represents the one having 6˜12 carbon atoms such as benzyl and naphthylmethyl.

Substituent groups in the foregoing substituted groups may include, for example, hydroxyl, carboxyl, carbamoyl, amino guanidino, imidazolyl, indolyl mercapto, and lower alkylthio.

R₆ and R₉, being the same or different from each other, each individually represents hydrogen, alkyl, aryl, or aralkyl. The alkyl, the aryl, and the aralkyl, being, for example, the foregoing ones, may further include alicylic compounds such as cyclopropyl, cyclopentyl, and cyclohexyl; and aromatic ring-condensed alicyclic compounds such as 2-indanyl and 1-indanyl.

R₁₂ represents hydroxyl, alkyloxy, aryloxy, aralkyloxy, amino, mono- or di-alkyl-, aryl-, or aralkylamino. The alkyloxy, the aryloxy, the aralkyloxy, the alkyl, the aryl, and the aralkyl may each individually include, for example, those cited above as examples of R₁ and R₂.

R₆ and R₇, combined together, may form an alkylene bridge having 2˜4 carbon atoms, an alkylene bridge having 2˜3 carbon atoms and 1 sulfur atom, an alkylene bridge having 3˜4 carbon atoms which contains a double bond, or substituted ones of these bridges; R₉ and R₁₀, combined together, may form the same alkylene bridges.

Examples of substituents on the foregoing bridges include hydroxyl, lower alkoxy, lower alkyl, oxo(o═), amino, condensed allyl, a condensed aromatic ring, and a condensed alicyclic ring.

Structures formed by such alkylene bridges may be, for example, the following ones: ##STR2##

Amino acids constituting the amino acid derivatives of the present invention may be either the L-isomer or the D-isomer.

The amino acid derivatives of the present invention may be in a form of salt, such as a metal salt, for example, sodium, potassium, lithium, and calcium salts; and a salt with an organic base. As the organic base, there can be adopted amines such as ammonia (ammonium salt), dicyclohexylamine, and N-methyl-D-glucamine; and basic amino acids such as lysine and arginine.

Needless to say, when the amino acid derivatives are used as ones to be included in antihypertensive drugs of the present invention, they need to be in a form of pharmaceutically acceptable salt.

The amino acid derivatives of the present invention are tripeptide derivatives of which the terminal amino group is combined with a phosphorus compound (P), being represented by the formula: P--X--Y--Z (X, Y, and Z each represents an amino acid residue).

The amino acid corresponding to X typically includes alanine, leucine, isoleucine, glycine, phenylalanine, arginine, glutamic acid, glutamin, lysine, valine, ornithine, methionine, serine, and threonine; the amino acid corresponding to Y typically includes proline, hydroxyproline, glycine, N-substituted glycine, and thioproline; the amino acid corresponding to Z typically includes proline, hydroxyproline, glycine, alanine, serine, aspartic acid, arginine, tyrosine, phenylalanine, valine, leucine, isoleucine, threonine, methionine, glutamic acid, glutamine, lysine, cystine, tryptophan, and histidine.

When the tripeptide derivative has functional groups, the functional groups may be protected by protecting groups ordinarily used in synthetic chemistry of peptides; the protected derivatives are also encompassed by the amino acid derivatives of the present invention.

Examples of methods for preparation are as follows: ##STR3## wherein R₁˜12 each individually represents the same as defined above.

The desired compound can be prepared by causing a phosphoridate [III] such as phosphochloridatediester and phosphochloridatemonoester to react with, for example, a hydrochloride derivative of ester or amide of the tripeptide derivative [II] in an inert solvent such as methylene chloride in the presence of a base such as triethylamine to transform the N-terminal of the tripeptide derivative into a phosphorus derivative, followed, if necessary, by selectively removing protecting groups by means of hydrolysis with an alkali, catalytic hydrogenation, or the like.

On the other hand, the desired compound can also be prepared by directly condensing a phosphoridate [III] with a free compound of the tripeptide derivative [II]. ##STR4## wherein R₁˜12 each individually represents the same as defined above.

The desired compound can be prepared by subjecting a phosphorus derivative of a dipeptide [IV] and an amino acid derivative [V] such as a hydrochloride of ester or amide to a condensation reaction in an inert solvent such as dimethylformamide (DMF) with the use of a condensing agent such as N,N'-dimethylaminopropylethylcarbodiimide (WSC) and 1-hydroxybenzotriazole (HOBt) to obtain a phosphorus derivative of the tripeptide [I], followed, if necessary, by selectively removing protecting groups by means of hydrolysis with an alkali or catalytic hydrogenation. ##STR5## wherein R₁˜12 each individually represents the same as defined above.

The desired compound can be obtained by subjecting a phosphorus derivative of an amino acid [VI] and a dipeptide derivative [VII] such as a hydrochloride of ester or amide to a condensation reaction in an inert solvent such as dimethylformamide with the use of a peptide condensating agent used in synthetic chemistry of peptides, such as WSC and HOBt, to obtain a phosphorus derivative of a tripeptide, followed, if necessary, by selectively removing protecting groups by means of hydrolysis with an alkali or catalytic hydrogenation.

The methods will be illustrated with more specific examples below.

A substituted phosphoryl derivative can be prepared by preparing a peptide derivative represented by the general formula: ##STR6## wherein Z represents the same as defined above, followed by causing the peptide derivative to react with a substituted phosphoryl halogenide such as diethyl- or dibenzylphosphoryl chloride. The substituted phosphoryl derivative, for example, dibenzylphosphoryl chloride, can be converted into a phosphoryl derivative by subjecting it to hydrogenation using palladium carbon as catalyst.

The foregoing tripeptide, an intermediate for the derivatives of the present invention, can be prepared by causing alanine having a protected amino group to react with proline having a protected carboxyl group to prepare alanylproline, removing the protecting groups of the alanylproline, and causing the resulting product to react with the Z component, an amino acid having a protected amino group, followed, if necessary, by removing the protecting group.

On the other hand, it can also be prepared by preparing dibenzylphosphorylalanylproline, followed by causing the product to react with the foregoing selected amino acid ester according to the method of Japanese patent first publication No. 104863/1981.

An alanylproline derivative of which the constituent proline residue has a hydroxyl group (hydroxyproline) can also be prepared in the same manner.

Those protecting groups for amino, imino, carboxyl, and hydroxyl groups, those methods for the protection, those methods for removal of the protecting groups, and those methods for amide-linking by condensation between amino and carboxyl groups which are all employed in the preparation of the derivatives of the present invention and intermediates therefore may be the ones ordinarily used in methods for peptide synthesis or generally or conventionally employed in the known literature, for example, Protein Chemistry, 1 Amino Acid Peptide, e.g. pages 405˜509 (1969), compiled by Shiro Akabori, Takeo Kaneko, and Kozo Narita, published by Kyoritsu Shuppan Co. An amide-linkage is advantageously formed from an amino acid by a condensation method in which an active ester of the amino acid having a protected amino group, such as p-nitrophenylester and N-hydroxysuccinimideester, is used for reaction. When a solvent is used in the reaction, DMF or water can be adopted as solvent. The reaction temperature may be a room temperature or so, but the reaction can also, as needed, be accelerated by heating.

The derivatives of the present invention are isolated from the reaction mixture by concentrating the reaction mixture to dryness, purifying the residue by means of column chromatography, followed by lyophilization of the product.

When the derivatives of the present invention are used, as an active ingredient, for an antihypertensive drug, there may be adopted their free forms, their nontoxic forms of salt, or their nontoxic forms having protecting groups. An amino acid constituting the derivatives for use as an antihypertensive drug of the present invention may be either the L-isomer or the D-isomer.

The amino acid derivatives of the present invention are useful as an antihypertensive drug for treating hypertensive mammals including humans. The derivatives can be used for lowering blood pressure by formulating them into a preparation such as tablets, capsules, and elixirs for oral administration and into an aspetic liquid preparation or an aseptic suspension preparation for parenteral administration. The amino acid derivatives of the present invention can be administered to a subject necessitating such treatment (animals and humans) in a dosage range of 0.2˜500 mg per subject generally several times a day, that is, in a total daily dosage of 1˜2000 mg. The dosage varies according to the seriousness of disease, the body weight of subjects, and other factors acknowledged by those skilled in the art.

The amino acid derivatives of the present invention can also be administered together with diuretics or other antihypertensive drugs. Typically, these drugs are administered in a dosage combination of which one unit of daily dose is in the range from 1/3 times as large as a clinical dosage minimally recommended to a level maximally recommended singly for each entity of disease. These combinations are specially shown as follows: A kind of antihypertensive drugs of the present invention which is clinically effective in a daily dosage range of 15˜200 mg can effectively be administered together with the following other antihypertensive drugs and diuretics in a daily dosage range of 3˜200 mg: hydrochlorothiazide (15˜200 mg), chlorothiazide (125˜2000 mg), ethacrynic acid (15˜200 mg), amiloride (5˜20 mg), furosemide (5˜80 mg), propranolol (20˜480 mg), timolol (5˜50 mg), methyldopa (65˜2000 mg). The foregoing dosage ranges are adjusted on the basis of unit according to the necessity for the possible daily divided dosage. The dosage varies according to the seriousness of disease, the body weight of subject, and other factors acknowledged by those skilled in the art.

The foregoing typical combinations of drugs are formulated into pharmaceutical compositions stated below. About 0.2˜500 mg of the derivatives of the present invention, pharmaceutically acceptable salt compounds, or mixtures of both are blended into unit dosage forms generally acknowledged or required for the pharmaceutical practice together with pharmaceutically acceptable vehicles, carriers, excipients, binders, antiseptics, stabilizers, flavorings, and so forth. The amount of each active substance in these compositions or preparations is adjusted in such a way as to give an appropriate dosage of the prescribed range.

Specific materials which can be incorporated into tablets, capsules, and so forth are as follows: A binder such as traganth, gum arabic, cornstarch, and gelatin; an excipient such as microcrystalline cellulose; a swelling agent such as cornstarch, pregelatinized starch, and arginic acid; a lubricant such as magnesium stearate; a sweetner such as sucrose, lactose, and saccharin; a flavoring such as peppermint, an oil from Gaultheria adenothrix Maxim, and cherry. When the unit dosage form of the preparation is a capsule, a liquid carrier such as fatty oil can further be incorporated in the foregoing type materials. Various other materials can be present as a coating material or in order to vary the physical form of unit dosage forms according to other methods. For example, tablets can be coated with shellac and/or sugar. Syrups or elixirs can contain active compounds, sucrose as a sweetner, methyl- and propylparaben as an antiseptic, a coloring matter, a flavoring such as cherry and an orange flavoring.

Aseptic compositions for injection can be formulated according to the usual practive for preparation of pharmaceutical dosage forms, in which practice an active substance is dissolved or suspended in a vehicle such as water for injection; natural vegetable oils such as sesame oil, palm oil, peanut oil, and cotton seed oil; and synthetic fat vehicle such as ethyl oleate. A buffer, an antiseptic, and an antioxidant can further be incorporated as occasion demands.

The present invention will be explained precisely in the following Examples.

EXAMPLE 1

Dibenzylphosphoryl-L-alanyl-L-prolyl-L-proline L-arginine salt:

A. Dibenzylphosphorylchloride

Phosphorus trichloride (3.7 g, 25 m mole) in benzene (15 ml) solution was added dropwise and slowly, to the mixture of dimethylaniline (6.1 g, 50 m mole) and benzylalcohol (5.4 g, 50 m mole) at a temperature of less than 15° C. by cooling with coolant of -15° C. and stirring. After the addition, the mixture was further stirred for 30 minutes and to the mixture benzylalcohol (2.7 g, 25 m mole) was further added dropwise. The mixture was reacted at a room temperature overnight.

The reaction solution was shaked with water (15 ml), and thus obtained organic phase was separated and then washed with water, 5N aqueous ammonia and water in order. The mixture was dried over anhydrous sodium sulfate. The drying agent was removed by filtration and the solvent was diStilled off under reduced pressure to give non-coloured and oily dibenzylhalogen phosphite (3.2 g).

Thus obtained oily material gave one spot by thin layer chromatography with silica gel (developing solvent; chloroform:ethanol:ethyl acetate=5:2:5, colour forming agent: Iodine).

Dibenzyl hydrogen phosphite (6.7 g) was dissolved in carbon tetrachloride (70 ml) dried previously, and put in a 3-neck flask. Sulfurylchloride (3 g) in carbon tetrachloride (10 ml) solution was added dropwise to the above mixture at a temperature of less than 10° C. while cooling with coolant of -15° C. and stirring and at an atmosphere of nitrogen gas. After the addition of sulfurylchloride, the mixture was stirred for 1.5 hours at room temperature at an atmosphere of nitrogen gas. It was confirmed that the starting material did not remain by tracing the reaction by thin layer chromatography (developing solvent; chloroform:ethanol:ethyl acetate=5:2:5, colour forming agent: Iodine). Thus produced dibenzylphosphoryl chloride without further purification was used in the following reaction.

B. N-t-butyloxycarbonyl-L-alanyl-L-proline benzyl ester

N-t-butyloxycarbonyl-L-alanine (3.8 g, 20 m mole), L-proline benzyl ester hydrochloride (5 g, 20.6 m mole) and 1-hydroxy benzotriazole, which is hereinafter referred to as HOBt (2.7 g, 20 m mole) were suspended in tetrahydrofuran, which is hereinafter referred to as THF (50 ml). N,N'-dimethylaminopropylethylcarbodiimide, which is hereinafter referred to as WSC (3.8 ml) in THF (10 ml) solution was added dropwisely to the above suspended solution while cooling with coolant of -15° C. and stirring. The reaction was carried for 3 hours at a temperature of less than 0° C. and thereafter at room temperature overnight. The solvent was removed by distillation under reduced pressure. The residue was dissolved in ethyl acetate and washed with 1N hydrochloric acid, water, 5% aqueous sodium bicarbonate and water in order, and dried over anhydrous sodium sulfate. The solvent was distilled off under reduced pressure. The residue was recrystallized in ether-n-hexan to give N-t-butyloxycarbonyl-L-alanyl-L-proline benzyl ester (6.7 g, yield: 89%) having melting point of 71° to 72° C. This product gave one spot by thin layer chromatography (developing solvent; chloroform:methanol:acetic acid=95:5:3, colour forming method; spraying with 0.1% ninhydrin and heating).

C. N-t-butyloxycarbonyl-L-alanyl-L-proline

N-t-butyloxycarbonyl-L-alanyl-L-proline benzyl ester (6.4 g, 17 m mole) was dissolved in methanol (100 ml). Hydrogen was passed for 3 hours through the solution in the presence of 10% palladium-carbon as a catalyst. The catalyst was removed by filtration and the solvent was distilled off under reduced pressure. The residue was crystallized in ethyl acetate-n-hexan to give N-t-butyloxycarbonyl-L-alanyl-L-proline (4.5 g, yield: 92.4%) having melting point of 155° to 157° C. and specific rotatory power [α]_(D) ²⁵ =-90.5° (C=1, ethanol). The product gave a single spot on thin layer chromatography.

D. N-t-butyloxycarbonyl-L-alanyl-L-prolyl-L-proline benzyl ester

N-t-butyloxycarbonyl-L-alanyl-L-proline (4.3 g, 15 m mole), L-proline benzyl ester hydrochloride (3.7 g, 15.3 m mole) and HOBt (2.0 g, 15 m mole) were dissolved in methylene dichloride (40 ml). WSC (2.8 ml) was added dropwise to the above mixture while cooling to -15° C. and stirring. The reaction was carried out for 3 hours at a temperature of not more than 0° C., and then overnight at room temperature. The solvent was removed by distillation under reduced pressure. The residue was dissolved in ethyl acetate, and washed with 1N hydrochloric acid, water, 5% sodium bicarbonate and water, in order. The mixture was dried over anhydrous sodium sulfate. The solvent was distilled off under reduced pressure. The residue was crystallized with a mixture of ethyl acetate and n-hexan to give a crystal of N-t-butyloxycarbonyl-L-alanyl-L-prolyl-L-proline benzyl ester (6.3 g, 88.7%) having melting point of 143° to 145° C. and specific rotatory power of [α]_(D) ²⁵ =-131.0° (C=1, chloroform).

E. Dibenzylphosphoryl-L-alanyl-L-prolyl-L-proline benzyl ester

N-t-butyloxycarbonyl-L-alanyl-L-prolyl-L-proline benzyl ester (6.0 g, 12.7 m mole) was dissolved in 4.8N hydrogen chloride in dioxan (15 ml) under shaking and stirred for 40 minutes at room temperature. The solvent was distilled off under reduced pressure and dried ethyl ether was added thereto. Thus obtained precipitate was obtained on the paper filter and soon put into the desiccator containing sodium hydroxide to be dried. All amount of the precipitate was dissolved in DMF (30 ml) and cooled to a temperature of -5° C. Triethyl amine was added to the cold mixture to neutralize it. Dibenzylphosphoryl chloride in carbon tetrachloride solution synthesized in the same manner described above and triethyl amine were slowly and dropwise added to the above neutralized solution at not more than 5° C. while cooling and stirring. In such case, the PH value of the reaction solution was always maintained from 8 to 9. After the completion of the addition, the reaction was carried out at room temperature overnight. The solvent was distilled off under reduced pressure. To thus obtained residue ethyl acetate (300 ml) was added and the mixture was washed with 1N hydrochloric acid, water, 5% sodium bicarbonate, and water in order and then dried with anhydrous sodium sulfate. The solvent was distilled off under reduced pressure to obtain oily dibenzylphosphoryl-L-alanyl-L-prolyl-L-proline benzyl ester (7.7 g, 96%). The oily material gave a single spot with R_(f) =0.65 by the thin layer chromatography (developing solvent; chloroform:methanol:acetic acid=95:5:3, colour forming method; spraying of 25% hydrobromic acid and 0.1% ninhydrine and heating).

F. Dibenzylphosphoryl-L-alanyl-L-prolyl-L-proline L-arginine salt

The oily material obtained above was dissolved in a mixture (20 ml) of acetone and methanol (1:1), and 1N sodium hydroxide (15 ml) was added thereto under cooling with ice-bath. The mixture was stirred for 1.5 hours at room temperature and was neutralized with 1N hydrochloric acid. The organic solvent was distilled off under reduced pressure. The residue was dissolved in ethyl acetate and washed with 1N hydrochloric acid and water, and dried with anhydrous sodium sulfate. The solvent was distilled off under reduced pressure to give oily dibenzylphosphoryl-L-alanyl-L-prolyl-L-proline (5.7 g). The oily material gave a single spot with R_(f) of 0.5 by the thin layer chromatography (developing solvent; chloroform:methanol:acetic acid=95:5:3, colour forming method; spraying of 25% hydrobromic acid and 0.1% ninhydrine, and heating).

The oily material (660 mg) was dissolved in ethanol (5 ml) and an aqueous L-arginine solution (1.2 ml) was added thereto. The arginine solution has been prepared by dissolving L-arginine in the water in a ratio of 1.74 g of L-argine in 10 ml of the solution.

The solvent was distilled off under reduced pressure, and then water was completely removed by azeotropic distillation with toluene. The residue was recrystalized with methanol and ethyl acetate to give the crystal of dibenzylphosphoryl-L-alanyl-L-prolyl-L-proline L-arginine salt (620 mg) having melting point 114° C. (s) to 122° C. (d) and specific rotatory power [α]_(D) ²⁰ =-73.3° (C=1.16, ethanol). A fixed weight of the sample of the product was heated in 6N hydrochloric acid at 110° C. for 17 hours, and from thus hydrolized material, amino acids analysis was carried out. The ratio of alanine, proline and arginine was 1.00:2.01:1.01.

Elementary analysis: Found C 50.65%, H 7.11%, N 12.30% Calculation C 50.17%, H 7.16%, N 12.41% as C₃₃ H₄₈ O₉ N₇ P.4H₂ O.

Median lethal dose (LD 50): more than 15 g/kg in mouse; more than 15 g/kg in rat.

EXAMPLE 2

Dibenzylphosphoryl-L-alanyl-L-prolyl-L-serine L-arginine salt:

Dibenzylphosphoryl-L-alanyl-L-proline (1.2 g, 2.5 m mole) as shown in the Japanese patent first publication No. 104863/1981, HOBt (338 mg, 2.5 m mole) and L-serine methylester hydrochloride (467 mg, 3 m mole) were suspended in DMF (10 ml), and WSC (0.5 ml) was added dropwise thereto while cooling to -15° C. and stirring. The reaction was carried out for 3 hours under cooling and next overnight at a room temperature. Ethyl acetate (100 ml) was added to the reaction solution, washed 1N hydrochloric acid and water in order, and dried with anhydrous sodium sulfate. The solvent was distilled off under reduced pressure to give an oily dibenzylphosphoryl-L-alanyl-L-prolyl-L-serine methyl ester (1.3 g). The oily product gave a single spot with R_(f) 0.46 by thin layer chromatography (developing solvent; chloroform:methanol:acetic acid=95:5:3, colour forming method: spraying of 25% hydrobromic acid and 0.1% ninhydrine and heating). The oily material was dissolved in a mixture (20 ml) of acetone and methanol (1:1) and 1N sodium hydroxide (5 ml) was added thereto under cooling with ice. The mixture was stirred for 1.5 hours at room temperature and neutralized with 1N hydrochloric acid. The organic solvent was distilled off under reduced pressure, and the residue was dissolved in ethyl acetate, washed with 1N hydrochloric acid and water in order, and dried with anhydrous sodium sulfate. The solvent was distilled off under reduced pressure to give oily dibenzylphosphoryl-L-alanyl-L-prolyl-L-serine (1.2 g). The oily material gave a single spot with R_(f) 0.18 by thin layer chromatography (developing solvent: chloroform:methanol:acetic acid=95:5:3, colour forming method; spraying of 25% hydrobromic acid and 0.1% ninhydrine, and heating).

The oily material (380 mg) was dissolved in ethanol (5 ml) and the aqueous L-arginine solution (0.71 ml) as prepared above. The solvent was distilled off under the reduced pressure, and residual water was removed completely by azeotropic distillation with toluene. To the residue, methanol was added and an insoluble material was separated by filtration. The solvent was distilled off under reduced pressure to give an amorphous powder of dibenzylphosphoryl-L-alanyl-L-prolyl-L-serine L-arginine salt (230 mg).

A fixed weight of the sample was heated in 6N hydrochloric acid at 110° C. for 19 hours. From this hydrolized product, amino acids analysis was carried out. The ratio of alanine, proline, serine and arginine was 1.00:1.02:0.96:1.07.

EXAMPLE 3

Dibenzylphosphoryl-L-alanyl-L-prolyl-L-aspartic acid L-arginine salt:

Dibenzylphosphoryl-L-alanyl-L-proline (1.2 g, 2.5 m mole), HOBt (338 mg, 2.5 m mole) and L-aspartic acid dibenzylester P-toluene sulfonic acid salt (1.45 g, 3 m mole) were suspended in DMF (10 ml), and WSC (0.5 ml) was added dropwise thereto while cooling at -15° C. and stirring. The reaction was carried out under cooling for 3 hours and thereafter at room temperature overnight. To the reaction solution ethyl acetate (100 ml) was added and thus obtained mixture was washed with 1N hydrochloric acid and water in order, and dried with anhydrous sodium sulfate. The solvent was distilled off under reduced pressure to obtain oily dibenzylphosphoryl-L-alanyl-L-prolyl-L-asparatic acid dibenzylester (1.9 g). The oily material gave a single spot with R_(f) 0.75 by thin layer chromatography (developing solvent; chloroform:methanol:acetic acid=95:5:3, colour forming method: spraying of 25% hydrobromic acid and 0.1% ninhydrine, and heating). This oily material was dissolved in a mixture (20 ml) of acetone and methanol (1:1), and 1 N sodium hydroxide (5 ml) was added thereto while cooling. Next, the mixture was stirred for 1.5 hours at room temperature and neutralized with 1N hydrochloric acid. The organic solvent was removed by distillation under reduced pressure, and the residue was dissolved in ethyl acetate, washed with 1N hydrochloric acid and water and dried with anhydrous sodium sulfate. The solvent was removed by distillation under reduced pressure to obtain oily dibenzylphosphoryl-L-alanyl-L-prolyl-L-aspartic acid (1.6 g). The oily material gave a single spot with R_(f) 0.15 by thin layer chromatography (developing solvent; chloroform:methanol:acetic acid=95:5:3, colour forming method: spraying of 25% hydrobromic acid and 0.1% ninhydrine, and heating).

The oily material (430 mg) was dissolved in ethanol (5 ml), and aqueous arginine solution (0.76 ml), which has been prepared by dissolving L-arginine in water in the ratio of 1.74 g of L-arginine per 10 ml of the aqueous arginine solution, was added thereto. The solvent was removed by distillation under reduced pressure, and then the water was removed completely by azeotropic distillation with toluene. Methanol was added to the residue, and thus prepared insoluble material was separated by filtration. The solvent was distilled off under reduced pressure to obtain amorphous powder of dibenzylphosphoryl-L-alanyl-L-prolyl-L-aspartic acid L-arginine salt (390 mg).

The fixed weight of the sample was heated in 6N hydrochloric acid at 110° C. for 19 hours. For thus obtained hydrolized material, amino acids analysis was carried out. The ratio of alanine, proline, aspartic acid and arginine was 1.00:0.97:1.04:1.04.

EXAMPLE 4

Dibenzylphosphoryl-L-alanyl-L-prolyl-L-tyrosine L-arginine salt:

Dibenzylphosphoryl-L-alanyl-L-proline (1.2 g, 2.5 m mole), HOBt (338 mg, 2.5 m mole) and L-tyrosine ethylester hydrochloride (737 mg, 3 m mole) was suspended in DMF (10 ml), and WSC (0.5 ml) was added gradually thereto while cooling to -15° C. and stirring. The reaction was carried out for 3 hours under cooling and then overnight at room temperature. To the reaction solution ethyl acetate (100 ml) was added, and the mixture was washed with 1N hydrochloric acid and water in order, and dried with anhydrous sodium sulfate. The solvent was removed by distillation under reduced pressure to obtain an oily dibenzylphosphoryl-L-alanyl-L-prolyl-L-tyrosine ethylester (1.4 g). This oily material gave a single spot with R_(f) 0.50 by thin layer chromatography (developing solvent; chloroform:methanol:acetic acid=95:5:3, colour forming method: spraying of 25% hydrobromic acid and 0.1% ninhydrine, and heating).

The oily material was dissolved in a mixture of acetone and methanol (1:1), and 1N sodium hydroxide (5 ml) was added thereto while cooling. Next, the mixture was stirred for 1.5 hours at room temperature, and neutralized with 1N hydrochloric acid. The organic solvent was distilled off under reduced pressure and the residue was dissolved in ethyl acetate, washed with 1N hydrochloric acid and water and dried with anhydrous sodium sulfate. The solvent was distilled off under reduced pressure to obtain oily dibenzylphosphoryl-L-alanyl-L-prolyl-L-tyrosine (1.2 g). The oily material gave a single spot with R_(f) 0.3 by thin layer chromatography (developing solvent; chloroform:methanol:acetic acid=95:5:3, colour forming method: spraying of 25% hydrobromic acid and 0.1% ninhydrine, and heating).

The oily material (390 mg) was dissolved in ethanol (5 ml), and aqueous arginine solution (0.64 ml) prepared as above mentioned was added thereto. The solvent was distilled off under reduced pressure and water was removed completely by azeotropic distillation with toluene. To the residue, methanol was added and thus obtained insoluble material was removed by filtration, and the solvent was distilled off to obtain an amorphous powder of dibenzylphosphoryl-L-alanyl-L-prolyl-L-tyrosine L-arginine salt (370 mg). A fixed weight of the sample (the product) was heated in 6N hydrochloric acid for 19 hours at 110° C., and amino acids in thus hydrolized material were analyzed. The ratio of alanine, proline, tyrosine and arginine was 1.00:0.99:1.03:1.02.

EXAMPLE 5

Dibenzylphosphoryl-L-alanyl-L-prolyl-L-phenylalanyl L-arginine salt:

Dibenzylphosphoryl-L-alanyl-L-proline (1.2 g, 2.5 m mole), HOBt (338 mg, 2.5 m mole) and L-phenylalanine ethylester hydrochloride (690 mg, 3 m mole) were suspended in DMF (10 ml) and WSC (0.5 ml) were added thereto while cooling to -15° C. and stirring. The reaction was carried out for 3 hours under cooling and then overnight at room temperature. To the reaction solution, ethyl acetate (100 ml) was added, and the mixture was washed with 1N hydrochloric acid and water in order, and dried with anhydrous sodium sulfate. The solvent was distilled off under reduced pressure to obtain oily dibenzylphosphoryl-L-alanyl-L-prolyl-L-phenylalanine ethylester (1.3 g). The oily material gave a single spot with R_(f) 0.7 by thin layer chromatography (developing solvent; chloroform:methanol:acetic acid=95:5:3, colour forming method: spraying of 25% hydrobromic acid and 0.1% ninhydrine, and heating).

The oily material was dissolved in a mixture (20 ml ) of acetone and methanol (1:1) and 1N sodium hydroxide (5 ml) was added under cooling. Next, the mixture was stirred for 1.5 hours at room temperature, and neutralized with 1N hydrochloric acid. The solvent was distilled off under reduced pressure and the residue was dissolved in ethyl acetate, washed with 1N hydrochloric acid and water, and dried with anhydrous sodium sulfate. The solvent was distilled off under reduced pressure to obtain an oily dibenzylphosphoryl-L-alanyl-L-prolyl-L-phenylalanine (0.9 g). The oily material gave a single spot with R_(f) 0.42 by thin layer chromatography (developing solvent; chloroform:methanol:acetic acid=95:5:3, colour forming method: spraying of 25% hydrobromic acid and 0.1% ninhydrine, and heating).

The oily material (280 mg) was dissolved in ethanol (5 ml), and the aqueous arginine solution (0.47 ml) as previously described was added thereto. The solvent was distilled off under reduced pressure and water was completely removed by azeotropic distillation with toluene. To the residue methanol was added and prepared insoluble matter was separated by filtration. The solvent was distilled off under reduced pressure to obtain dibenzylphosphoryl-L-alanyl-L-prolyl-L-phenylalanine L-arginine salt (270 mg). The fixed weight of the sample was heated in 6N hydrochloric acid for 19 hours at 110° C. In the thus hydrolized material amino acids were analyzed. The ratio of alanine, proline, phenylalanine and arginine was 1.00:1.00:1.08:1.05.

EXAMPLE 6

Dibenzylphosphoryl-L-alanyl-L-prolyl-glycine L-arginine salt:

Dibenzylphosphoryl-L-alanyl-L-proline (1.2 g, 2.5 m mole), HOBt (338 mg, 2.5 m mole) and glycine benzylester p-toluene sulfonic acid salt (1.0 g, 3 m mole) were suspended in DMF (10 ml), and WSC (0.5 ml) was dropwise added thereto while cooling to -15° C. and stirring. The reaction was carried out for 3 hours under cooling, and next, overnight at room temperature. To the reaction solution ethyl acetate (100 ml) was added and the mixture was washed with 1N hydrochloric acid and water in order, and dried with anhydrous sodium sulfate. The solvent was distilled off under reduced pressure to give oily dibenzylphosphoryl-L-alanyl-L-prolyl-glycine benzylester-(1.5 g). The oily material gave a single spot with R_(f) 0.7 by thin layer chromatography (developing solvent; chloroform:methanol:acetic acid=95:5:3, colour forming method: spraying of 25% hydrobromic acid and 0.1% ninhydrine and heating).

This oily material was dissolved in a mixture (20 ml) of acetone and methanol (1:1) and 1N sodium hydroxide (5 ml) was added thereto under cooling. Next, the mixture was stirred for 1.5 hours at room temperature, and neutralized with 1N hydrochloric acid. The organic solvent was distilled off under reduced pressure, and the residue was dissolved in ethyl acetate, washed with 1N hydrochloric acid and water in order, and dried with anhydrous sodium sulfate. The solvent was distilled off under reduced pressure to obtain oily dibenzylphosphoryl-L-alanyl-L-prolylglycine (1.4 g). The oily material gave a single spot with R_(f) 0.4 by thin layer chromatography (developing solvent; chloroform:methanol:acetic acid=95:5:3, colour forming method: spraying of 25% hydrobromic acid and 0.1% ninhydrine and heating).

The oily material (610 mg) was dissolved in ethanol (5 ml) and the aqueous arginine solution as previously described (1.2 ml) was added thereto. The solvent was distilled off under reduced pressure, and further water was removed completely by azeotropic distillation with toluene. To the residue methanol was added and the insoluble material was separated by filtration. The solvent was distilled off under reduced pressure to obtain an amorphous powder of dibenzylphosphoryl-L-alanyl-L-prolyl-glycine L-arginine salt (570 mg). The fixed weight of the sample was heated in 6N hydrochloric acid for 19 hours at 110° C. In the hydrolized material, amino acids were analyzed. The ratio of alanine, proline, glycine and arginine was 1.00:0.91:1.00:1.11.

EXAMPLE 7

Dibenzylphosphoryl-L-alanyl-L-prolyl-L-valine L-arginine salt:

By using L-valine benzylester hydrochloride (15.3 m mole) in or L-glutamic acid benzylester hydrochloride (15.3 m mole) in place of L-proline benzylester hydrochloride (3.7 g, 15.3 m mole) as used in Example 1, the same experiment as in Example 1 is repeated and thereby dibenzylphosphoryl-L-alanyl-L-prolyl-L-valine L-arginine salt can be produced.

EXAMPLE 8

Phosphoryl-L-alanyl-L-prolyl-L-proline L-arginine salt:

Dibenzylphosphoryl-L-alanyl-L-prolyl-L-proline L-arginine salt (3 g, 4.2 m mole), and L-arginine (1.45 g, 8.36 m mole) were dissolved in a mixture of ethanol (50 ml) and water (10 ml), and palladium-carbon (1 g) was added thereto. After that catalytic reduction reaction was carried out for 5 hours. The catalyst was removed by filtration and the solvent was removed by distillation. The residue was dissolved in water and freeze-dried to obtain phosphoryl-L-alanyl-L-prolyl-L-proline tri-L-arginine salt (3.63 g). After hydrolysis of the material with 6N hydrochloric acid, amino acids were analyzed. The ratio of alanine, proline and arginine was 1.00:1.99:2.90.

EXAMPLE 9

Dibenzylphosphoryl-L-alanyl-L-prolyl-L-leucine L-arginine salt:

By using L-leucine benzylester hydrochloride (15.3 m mole) in place of L-proline benzylester hydrochloride (3.7 g, 15.3 m mole) as used in Example 1, the same experiment as in Example 1 is repeated, and thereby dibenzylphosphoryl-L-alanyl-L-prolyl-L-leucine L-arginine salt can be produced.

EXAMPLE 10

Dibenzylphosphoryl-L-alanyl-L-prolyl-L-threonine,-L-methionine or -L-glutamic acid L-arginine salt:

By using hydroxyl group-protected-L-threonine, L-methionine or L-glutamic acid benzylester hydrochloride (15.3 m mole) in place of L-proline benzylester hydrochloride (3.7 g, 15.3 m mole) as used in Example 1, the same experiments as in Example 1 are repeated and the protective group is removed, if necessary, and thereby the titled objective amino acid derivatives can be produced.

EXAMPLE 11

Dibenzylphosphoryl-L-alanyl-L-prolyl-L-proline amide:

Dibenzylphosphoryl-L-alanyl-L-proline (2.8 g, 6.4 m mole), HOBt (952 mg, 7 m mole), and L-proline amide (805 mg, 7 m mole) were dissolved in DMF (10 ml), and N,N'-dimethylaminopropylethylcarbodiimide hydrochloride (1.34 g, 7 m mole) was added thereto while cooling to -15° C.

The reaction was continued for 3 hours under cooling and then overnight at room temperature, DMF was distilled by reduced pressure, and the residue was dissolved in chloroform. The mixture was washed with water, dried with anhydrous sodium sulfate, and the solvent was removed by distillation.

The residue was reprecipitated with methanol-ethyl ether to obtain dibenzylphosphoryl-L-alanyl-L-prolyl-L-prolineamide (890 mg). Melting point: 89° C. The specific rotatory power [α]_(D) ²⁰ =-29.9° (C=0.4, 1N acetic acid). After hydrolysis of the product with 6N hydrochloric acid, the amino acids were analyzed. The ratio of alanine, proline and ammonia was 1.00:0.99:1.05.

EXAMPLE 12

Dibenzylphosphoryl-L-alanyl-L-prolyl-L-alanine L-arginine

salt:

Dibenzylphosphoryl-L-alanyl-L-proline (1.4 g, 3.2 m mole), 1-hydroxybenzotriazol (476 mg, 3.5 m mole) and L-alanine benzylester p-toluene sulfonate (1.3 g, 3.5 m mole) were dissolved in DMF (5 ml), and WSC (0.64 ml) was added thereto while cooling to -15° C. The reaction was continued for 3 hours under cooling and then overnight at room temperature, and ethyl acetate (100 ml) was added thereto. The mixture was washed with 1N hydrochloric acid, 5% sodium bicarbonate and water in order, and dried with anhydrous sodium sulfate. The solvent was distilled off under reduced pressure to obtain an oily dibenzylphosphoryl-L-alanyl-L-prolyl-L-alanine benzylester.

Thus obtained oily material was dissolved in acetone (25 ml) and 1N sodium hydroxide (4 ml) was added thereto while cooling with ice. Next, the mixture was stirred for 1 hour at room temperature and neutralized with 1N hydrochloric acid. The solvent was distilled off under reduced pressure, and the residue was dissolved in ethyl acetate. The mixture was washed with 1N hydrochloric acid and water in order, and dried anhydrous sodium sulfate. The solvent was distilled off under reduced pressure, and thus obtained oily material was dissolved in ethanol (3 ml). The aqueous arginine solution (1.2 ml) as described above, was added thereto, and the solvent was distilled off under reduced pressure. Further, water was removed completely by azeotropic distillation with toluene. To the residue, ethanol was added and insoluble material was separated by filtration. By adding ethyl acetate to the filtrate, an amorphous powder (500 mg) was obtained. Melting point was 110°˜120° C.

Elementary analysis: Found C 52.11%, H 7.09%, N 13.69% Calculation C 52.19%, H 6.81%, N 13.74% as C₃₁ H₄₆ N₁₁ O₉ P. 1.2H₂ O

After hydrolysis of the product with 6N hydrochloric acid, amino acids were analyzed. The ratio of alanine, proline and arginine was 1.94:1.03:1.00.

EXAMPLE 13

Dibenzylphosphoryl-L-alanyl-L-prolyl-L-arginine:

Dibenzylphosphoryl-L-alanyl-L-proline (1.8 g, 4 m mole), HOBt (540 mg), and L-arginine methylester 2 hydrochloric acid salt (2.62 g, 10 m mole) were dissolved in DMF (30 ml), and then triethylamine (2.8 ml, 20 m mole) and dicyclohexylcarbodiimide (1.03 g) were added thereto while cooling to -15° C. The reaction was carried out for 4 hours under cooling and then overnight at room temperature. Thus produced dicyclohexylurea was separated by filtration. The solvent was distilled off and 0.1N hydrochloric acid was added to the residue. The mixture was washed with ethyl ether and the aqueous layer was absorbed with ion exchanging resin "Dia ion HP-20" as produced by Mitsubishikaseikogyo Co., Inc. The resin was washed with water and the desired substances were eluted with 80% methanol (methanol: 5% acetic acid=80:20). The eluants were concentrated and the thus obtained residue was dissolved in acetone (10 ml). 0.1N sodium hydroxide (5 ml) was added to the mixture under cooling and the mixture was stirred for 1 hour at room temperature. The mixture was neutralized with 1N hydrochloric acid and the solvent was distilled off under reduced pressure. The residue was dissolved in water and treated with ion exchanging resins "Dia ion HP-20" for adsorption of the desired substances. The resin was washed with water and the desired substances were eluted with 80% methanol, which is the same composition as above. The eluants were concentrated and the thus obtained residue was dissolved in ethanol. To the solution ethyl ether was added to give an amorphous powder of dibenzylphosphoryl-L-alanyl-L-prolyl-L-arginine (800 mg). The specific rotatory power was [α]_(D) ²⁰ =-36.5° (C=0.5, ethanol).

Elementary analysis: Found C 54.60%, H 6.81%, N 13.54% Calculation C 54.66%, H 6.62%, N 13.66% as C₂₈ H₃₉ N₆ O₇ P.0.7H₂ O

After hydrolysis of the product with 6N hydrochloric acid, amino acids were analyzed. Alanine:Proline:Arginine=1.00:1.01:1.00.

EXAMPLE 14

Dibenzylphosphoryl-L-alanyl-L-prolyl-D-alanine L-arginine salt:

Dibenzylphosphoryl-L-alanyl-L-proline (1.4 g, 3.2 m mole), HOBt (476 mg, 3.5 m mole) and D-alanine benzylester p-toluene sulfonic acid salt (1.3 g, 3.5 m mole) were dissolved in DMF (5 ml), and WSC (0.64 ml) was added thereto while cooling to -15° C. The reaction was carried out for 3 hours under cooling and then overnight at room temperature. To the reaction solution ethyl acetate (100 ml) was added and the mixture was washed with 1N hydrochloric acid, 5% sodium bicarbonate and water in order. The mixture was dried with anhydrous sodium sulfate and the solvent was distilled off under reduced, pressure to give oily dibenzylphosphoryl-L-alanyl-L-prolyl-D-alanine benzylester.

Thus obtained oily material was dissolved in acetone (25 ml) and 1N sodium hydroxide (4 ml) was added under cooling. Next, the mixture was stirred for 1 hour at room temperature, and then neutralized with 1N hydrochloric acid. The solvent was removed by distillation under reduced pressure and the residue was dissolved in ethyl acetate. The mixture was washed with 1N hydrochloric acid-and water, and dried with anhydrous sodium sulfate. The solvent was distilled off. The residue was dissolved in ethyl ether and n-hexan was added thereto to precipitate a crystal. The crystal was dissolved in ethanol and the aqueous arginine solution as mentioned above (1.2 ml) was added. The solvent was distilled off under reduced pressure and further water was completely removed by azeotropic distillation with toluene. To the residue ethanol was added and thus produced insoluble material was separated by filtration. To the filtrate ethyl ether was added to give a crystal of dibenzylphosphoryl-L-alanyl-L-prolyl-D-alanine L-arginine salt (1.3 g). Melting point: 82°˜85° C., Specific rotatory power [α]_(D) ²⁰ =-31.1° (C=0.5, ethanol).

Elementary analysis: Found C 51.70%, H 6.93%, N 13.79% Calculation C 51.80%, H 6.87%, N 13.64% as C₃₁ H₄₆ N₇ O₉ P.1.5H₂ O

After hydrolysis of the product with 6N hydrochloric acid, amino acids were analyzed. Alanine:Proline:Arginine=2.00:1.03:0.97.

EXAMPLE 15

Dibenzylphosphoryl-L-alanyl-L-prolyl-D-proline L-arginine salt:

Dibenzylphosphoryl-L-alanyl-L-proline (1.4 g, 3.2 m mole), HOBt (476 mg, 3.5 m mole) and oily D-proline benzylester hydrochloride (968 mg, 4 m mole) were dissolved in DMF (5 ml) and WSC (0.64 ml, 3.5 m mole) were added thereto while cooling to -15° C. The reaction was carried out for 3 hours under cooling and then overnight at room temperature, and ethyl acetate (100 ml) was added to the reaction solution. The mixture was washed with 1N hydrochloric acid, 5% sodium bicarbonate and water in order, and then dried with anhydrous sodium sulfate. The solvent was distilled off under reduced pressure to obtain an oily dibenzylphosphoryl-L-alanyl-L-prolyl-D-proline benzylester.

Thus obtained oily material was dissolved in acetone (20 ml) and 1N sodium hydroxide (4 ml) was added thereto under cooling. The mixture was stirred for 2 hours at room temperature, and neutralized with 1N hydrochloric acid. The solvent was distilled off under reduced pressure and the residue was dissolved in ethyl acetate. The mixture was washed with 1N hydrochloric acid and water in order, and then dried with anhydrous sodium sulfate. The solvent was distilled off under reduced pressure, and the residue was dissolved in ethanol (3 ml), and the aqueous arginine solution as mentioned above (1.2 ml) was added thereto. The solvent was distilled off under reduced pressure, and further, water was completely removed by azeotropic distillation with toluene. To the residue ethanol was added and insoluble material was separated by filtration. By adding ethyl ether to the filtrate, an amorphous powder of dibenzylphosphoryl-L-alanyl-L-prolyl-D-proline L-arginine salt (585 mg) was obtained.

Elementary Analysis Found C 50.43%, H 7.09%, N 12.39%, Calculation C 50.17%, H 7.16%, N 12.41% as C₃₃ H₄₈ N₇ O₉ P. 4H₂ O

After hydrolysis of the product with 6N hydrochloric acid, amino acids were analyzed.

Alanine:Proline:Arginine=1.00:2.08:1.01.

EXAMPLE 16

Dibenzylphosphoryl-L-alanyl-L-prolyl-trans-L-hydroxyproline L-arginine salt:

Dibenzylphosphoryl-L-alanyl-L-proline (1.4 g, 3.2 m mole), HOBt (476 mg, 3.5 m mole), and trans-L-hydroxyproline benzylester p-toluene sulfonic acid salt (1.4 g, 3.5 m mole) were dissolved in DMF, and WSC (0.64 ml, 3.5 m mole) was added thereto while cooling to -15° C. The reaction was carried out for 3 hours under cooling and then overnight at room temperature. To the reaction solution ethyl acetate (100 ml) was added and the mixture was washed with 1N hydrochloric acid, 5% sodium bicarbonate and water in order, and dried with anhydrous sodium sulfate. The solvent was distilled off under reduced pressure to obtain oily dibenzylphosphoryl-L-alanyl-L-prolyl-trans-L-hydroxyproline benzylester.

Thus obtained oily material was dissolved in acetone (15 ml), and 1N sodium hydroxide (4 ml) was added thereto under cooling with ice. The mixture was stirred for 2 hours at room temperature and neutralized with 1N hydrochloric acid.

The solvent was distilled off under reduced pressure and the residue was dissolved in water, and washed with ethyl acetate. The water solution was treated with ion exchanging resin "Dia ion HP-20" for adsorption of desired product. The resin was washed with water and the desired product was eluated with 80% methanol (methanol:5% acetic acid=80:20). The eluate was concentrated under reduced pressure and the residue was dissolved in ethanol, and the aqueous arginine solution as mentioned above (1.2 ml) was added thereto. The solvent was distilled off under reduced pressure and further water was completely removed by azeotropic distillation with toluene. To the residue ethanol was added and isoluble material was separated by filtration. Ethyl ether was added to the filtrate to obtain an amorphous powder of dibenzylphosphoryl-L-alanyl-L-prolyl-trans-L-hydroxyproline L-arginine salt (450 mg). After hydrolysis of the product with 6N hydrochloric acid, amino acids were analyzed. Alanine:Proline:Hydroxyproline:Arginine=1.00:1.04:0.98:0.78.

EXAMPLE 17

Dibenzylphosphoryl-L-alanyl-trans-L-hydroxyprolyl-L-proline:

A. t-butyloxycarbonyl-L-alanyl-trans-L-hydroxyproline

t-Butyloxycarbonyl-L-alanine (2.84 g, 1.5 m mole) and trans-L-hydroxyproline benzylester p-toluene sulfonic acid salt (5.91 g, 15 m mole) were dissolved in methylene dichloride (50 ml) and WSC (2.75 ml, 15 m mole) was added thereto while cooling to -15° C. The reaction was carried out for 2 hours under cooling and then for 3 hours at room temperature, and the methylene dichloride was distilled off under reduced pressure. The residue was dissolved in ethyl acetate (100 ml) and washed with 1N hydrochloric acid, 5% sodium bicarbonate and water in order, and dried with anhydrous sodium sulfate. The solvent was distilled off under reduced pressure to obtain oily t-butyloxycarbonyl-L-alanyl-trans-L-hydroxyproline benzylester.

The thus obtained oily material was dissolved in methanol (100 ml), and palladiu-m-carbon (500 mg) was added thereto. The reductive reaction was carried out for 5 hours. The catalyst was removed by filtration and the solvent was distilled off under reduced pressure. The residue was recrystalized with ethyl acetate-hexan to give t-butyloxycarbonyl-L-alanyl-trans-L-hydroxyproline (3.7 g).

B. t-butyloxycarbonyl-L-alanyl-trans-L-hydroxyprolyl-L-proline benzylester

t-Butyloxycarbonyl-L-alanyl-trans-L-hydroxyproline (3.02 g, 10 m mole) and L-proline benzylester hydrochloride (2.66 g, 11 m mole) were dissolved in methylene dichloride (50 ml) and WSC (2.01 ml, 11 m mole) was added thereto while cooling to -15° C. The reaction was carried out for 3 hours at room temperature. The methylene dichloride was distilled off under reduced pressure and the residue was dissolved in ethyl acetate (100 ml), and washed with 1N hydrochloric acid, 5% sodium bicarbonate and water in order, and dried with anhydrous sodium sulfate. The solvent was distilled off under reduced pressure and the residue was recrystalized with ethyl acetate-n-hexan to obtain t-butyloxycarbonyl-L-alanyl-trans-L-hydroxyprolyl-L-proline benzylester (4.1 g).

C Dibenzylphosphoryl-L-alanyl-L-trans-L-hydroxyprolyl-L-proline

t-Butyloxycarbonyl-L-alanyl-L-trans-L-hydroxyprolyl-L-proline benzylester (2.93 g, 6 m mole) and trifluoroacetic acid (10 ml) were mixed, and stirred for 40 minutes. Trifluoroacetic acid was distilled off under reduced pressure, and 4.8N hydrochloric acid dioxane solution (1.88 ml, 9 m mole) was added to the residue. The mixture was stirred well and ethyl ether was added thereto.

Thus produced precipitate was obtained on the filter paper and dried over sodium hydroxide in the desiccator. The precipitate was dissolved in DMF (3 ml) and neutralized by adding triethylamine (1.4 ml) while cooling to -5° C. Dibenzylphosphorylchloride carbon tetrachloride solution (10 m mole) and triethylamine were added thereto at not more than 5° C. and at pH 8 to 9. The mixture was stirred for three hours and the solvent was distilled off under reduced pressure, The residue was dissolved in ethyl acetate (100 ml) and washed with 1N hydrochloric acid, 5% sodium bicarbonate and water in order, and dried with anhydrous sodium sulfate. The solvent was removed by distillation under reduced pressure to obtain dibenzylphosphoryl-L-alanyl-trans-L-hydroxyprolyl-L-proline benzylester.

Thus obtained oily material was dissolved in methanol (20 ml) and 1N sodium hydroxide (6 ml) was added thereto while cooling with ice, and stirred for 3 hours at room temperature. The mixture was neutralized with 1N hydrochloric acid, and the solvent was distilled off under reduced pressure. The residue was dissolved in water and treated with ion exchanging resin "Dia ion HP-20" for adsorption of the desired product. The resin was washed with water, and the desired product was eluated with 80% methanol. The eluate was concentrated and the residue was recrystalized with ethyl acetate-n-hexan to obtain dibenzylphosphoryl-L-alanyl-trans-L-hydroxyprolyl-L-proline (650 mg). After hydrolysis of the product with 6N hydrochloric acid, amino acids were analyzed. Alanine:Proline:Hydroxyproline=1.00:0.99:0.99.

EXAMPLE 18

Dibenzylphosphoryl-L-isoleucyl-L-prolyl-L-proline L-arginine salt:

The oily t-Butyloxycarbonyl-L-isoleucyl-L-prolyl-L-proline benzylester (10 g, 20 m mole) and trifluoroacetic acid (50 ml) were mixed together under cooling, and stirred for 50 minutes. The trifluoroacetic acid was distilled off under reduced pressure, and the residue was dissolved in DMF (50 ml). The mixture was neutralized with triethylamine (3.5 ml) under cooling to -5° C., and thereafter dibenzylphosphorylchloride carbontetrachloride solution (30 m mole) and triethylamine were dropwise added thereto at not more than 5° C. and at the pH range 8-9. The mixture was stirred for 3 hours at room temperature and the solvent was distilled off under reduced pressure. The residue was dissolved in ethyl acetate (300 ml) and washed with 1N hydrochloric acid, 5% sodium bicarbonate and water in order, and dried with anhydrous sodium sulfate. The solvent was distilled off under reduced pressure to obtain an oily dibenzylphosphoryl-L-isoleucyl-L-prolyl-L-proline benzylester.

All the amounts of the oily material was dissolved in acetone (100 ml) and 1N sodium hydroxide (25 ml) was added thereto under cooling with ice and the mixture was stirred for 2 hours at room temperature. The mixture was neutralized with 1N hydrochloric acid, and the solvent was distilled off under reduced pressure. The residue was dissolved in ethyl acetate. The mixture was washed with 1N hydrochloric acid and water in order, and dried with anhydrous sodium sulfate and) the solvent was distilled off under reduced pressure. The residue was dissolved in ethanol, and the solution prepared by dissolving L-arginine (1.3 g) in water (3 ml) was added, and the resulting insoluble material was separated by filtration. From the filtrate the solvent was distilled off under reduced pressure.

The residue was recrystalized with ethanol-ethyl acetate to obtain dibenzylphosphoryl-L-isoleucyl-L-prolyl-L-proline L-arginine salt (4.3 g). Melting point: 141° to 148° C. A specific rotatory power: [α]_(D) ²⁰ =-84.9° (C=0.5, 50% ethanol).

Elementary analysis Found C 53.28%, H 7.20%, N 12.65% Calculation C 53.11%, H 7.44%, N 12.05% as C₃₆ H₅₄ O₉ N₇ P.3H₂ O

After hydrolysis of the product with 6N hydrochloric acid, amino acids were analyzed. Isoleucine:Proline:Arginine=1.03:2.00:0.99

EXAMPLE 19

Dibenzylphosphoryl-glycyl-L-prolyl-L-proline L-arginine salt:

Trifluoroacetic acid (10 ml) was added to t-butyloxycarbonyl-glycyl-L-prolyl-L-proline (1.85 g, 5 m mole) while cooling and the mixture was stirred for 40 minutes. The trifluoroacetic acid was distilled off under reduced pressure, and to the residue ethyl ether was added. Thus produced precipitate was obtained on the filter paper, and dissolved in DMF (10 ml). The mixture was neutralized by adding triethylamine (1.5 ml) to the mixture while cooling to -5° C., and dibenzylphosphorylchloride carbon tetrachloride solution (7 m mole) and triethylamine were dropwise added thereto while keeping a temperature not more than 5° C., and keeping a pH range to 8-9. The mixture was stirred for 3 hours at room temperature. The solvent was distilled off and the residue was dissolved in ethyl acetate (100 ml) and washed with 1N hydrochloric acid and water in order, and dried with anhydrous sodium sulfate. The solvent was distilled off and the residue was dissolved in ethanol, and L-arginine (690 mg) water (2 ml) solution was added thereto. Water was removed by distillation of the solvent and by azeotropic distillation with toluene. To the residue ethanol was added and insoluble material was separated by filtration. Ethyl ether was added to the filtrate to obtain dibenzylphosphorylglycyl-L-prolyl-L-proline L-arginine salt (430 mg).

After the hydrolysis of the product, amino acids were analyzed, Glycine:Proline:Arginine=1.00:1.96:0.96.

EXAMPLE 20

Dibenzylphosphoryl-L-phenylalanyl-L-Prolyl-L-proline:

t-Butyloxycarbonyl-L-phenylalanyl-L-prolyl-L-proline benzylester (1.65 g, 3 m mole) and trifluoroacetic acid (10 ml) were mixed together for 40 minutes under cooling. The trifluoroacetic acid was distilled off under reduced pressure, and the residue was dissolved in DMF (5 ml). The mixture was neutralized by adding triethylamine (0.8 ml) thereto while cooling to -5° C., and further dibenzylphosphorylchloride carbon tetrachloride solution (5 m mole) were added dropwise while keeping a temperature to not more than 5° C. The pH range of reaction solution was usually kept from 8 to 9 by adding triethylamine. The mixture was stirred for 3 hours at room temperature, and then the solvent was distilled off. The residue was dissolved in ethyl acetate (100 ml) and washed with 1N hydrochloric acid, 5% sodium bicarbonate and water in order, and dried with anhydrous sodium sulfate. The solvent was distilled off under reduced pressure to obtain oily dibenzylphosphoryl-L-phenylalanyl-L-prolyl-L-proline benzylester. The oily material was dissolved in acetone (20 ml) and 1N sodium hydroxide (4 ml) was added thereto while cooling with ice and the mixture was stirred for 3 hours at room temperature. After neutralization of the mixture with 1N hydrochloric acid, the solvent was distilled off under reduced pressure. The residue was dissolved in ethyl acetate. The mixture was dried with 1N hydrochloric acid and water in order, and dried with anhydrous sodium sulfate. The solvent was distilled off under reduced pressure, and the residue was reprecipitated with ethyl acetate and n-hexan to obtain dibenzylphosphoryl-L-phenylalanyl-L-prolyl-L-proline (820 mg).

After hydrolysis of the product with 6N hydrochloric acid, amino acids were analyzed. Phenylalanine:Proline=0.99:2.00.

EXAMPLE 21

Dibenzylphosphoryl-L-arginyl-L-prolyl-L-proline

Anisole (0.5 ml) was added to amyloxycarbonyl-tosyl-L-arginyl-L-prolyl-L-proline benzylester (1.45 g, 2 m mole) and anhydrous hydrogen fluoride (20 ml) was added thereto while cooling to -40° C. The reaction was carried out for 60 minutes at 0° C., and anhydrous hydrogen fluoride was distilled off under reduced pressure. The residue was washed with ethyl ether, and dissolved in 1N acetic acid. The mixture was passed through adsorbent "Dowex 1×2" (type of CH₃ COO⁻) as produced by Dow Chemical Co. and the desired product was eluated with water. The eluate was freeze-dried. Thus obtained amorphous L-arginyl-L-prolyl-L-proline was dissolved in DMF (8 ml) and water (2 ml), and triethylamine (0.3 ml) was added thereto while cooling to -5° C. for neutralization.

To the mixture dibenzylphosphorylchloride carbon tetrachloride solution (3 m mole) and triethylamine were added dropwise while keeping the temperature to not more than 5° C., and keeping usually pH value to 8-9. The mixture was stirred for 3 hours at room temperature. The solvent was distilled off under reduced pressure, and the residue was dissolved in water and then passed through "Dowex 1×2" (a type of CH₃ COO⁻). The desired product was eluated with water and the thus obtained eluate was freeze-dried. The thus obtained amorphous powder was further purified by column chromatography with silica gel (eluating solution; chloroform:methanol:acetic acid=85:15:5). Main ingredients were collected and freeze-dried to obtain amorphous powder of dibenzylphosphoryl-L-arginyl-L-prolyl-L-proline (270 mg).

After hydrolysis of the product with 6N hydrochloric acid, amino acids were analyzed. Arginine:Proline=1.04:2.00.

EXAMPLE 22

Dibenzylphosphoryl-L-glutamyl-L-prolyl-L-proline

Anisole (0.5 ml) was added to t-butyloxycarbonyl-γ-benzyl-L-glutamyl-L-prolyl-L-proline (1.06 g, 2 m mole) and anhydrous hydrogen fluoride (20 ml) was added thereto while cooling -40° C. The reaction was carried out for 1 hour at 0° C., and then anhydrous hydrogen fluoride was distilled off under reduced pressure. The residue was washed with ethyl ether and then dissolved in 1N acetic acid, and the mixture was passed through ion exchanging resin "IR-45" (CH₃ COO⁻). The desired product was eluated with water and the eluate was freeze-dried.

The thus obtained amorphous L-glutamyl-L-prolyl-L-proline was dissolved in DMF (8 ml) and water (2 ml), and neutralized by addition of triethylamine (0.2 ml) while cooling to -5° C. Dibenzylphosphorylchloride carbon tetrachloride solution (3 m mole) and triethylamine were added dropwise to the mixture while keeping the temperature to not more than 5° C. and usually keeping the pH value to 8-9. The mixture was stirred for 3 hours at room temperature, and the solvent was distilled off under reduced pressure. The residue was dissolved in 5% sodium bicarbonate and then washed with ethyl acetate. The aqueous phase was acidified with 1N hydrochloric acid, and the desired product was extracted with ethyl acetate. The ethyl acetate phase was washed with sodium chloride solution and dried with anhydrous sodium sulfate. The ethyl acetate was distilled off under reduced pressure. The residue was re-precipitated with ethyl acetate-ethyl ether to obtain dibenzylphosphoryl-L-glutamyl-L-prolyl-L-proline (140 mg).

After hydrolysis of the product with 6N hydrochloric acid, amino acids were analyzed. Glutamic acid:Proline=0.92:2.00.

EXAMPLE 23

Diethylphosphoryl-L-lysyl-L-prolyl-L-proline L-arginine salt:

A. t-Butyloxycarbonyl-ε-carbobenzoxy-L-lysyl-L-proline benzylester

t-Butyloxycarbonyl-ε-carbobenzoxy-L-lysine dicylohexylamine salt (10 g, 17.8 m mole) was suspended in ethyl acetate, and washed with 1N sulfuric acid. Ethyl acetate phase was washed with water and dried with anhydrous sodium sulfate, and ethyl acetate was distilled off under reduced pressure.

All the obtained oily t-butyloxycarbonyl-ε-carbobenzoxy-L-lysine and L-proline benzylester hydrochloride (4.3 g, 17.8 m mole) were dissolved in methylene dichloride and WSC (3.3 ml, 17.8 m mole) was added thereto while cooling to -15° C. The mixture was stirred overnight and the methylene dichloride was distilled off under reduced pressure. The residue was dissolved in ethyl acetate. The ethyl acetate solution was washed with 1N hydrochloric acid, 5% sodium bicarbonate and water in order, and dried with anhydrous sodium sulfate. The ethyl acetate was distilled off under reduced pressure to obtain an oily t-butyloxycarbonyl-ε-carbobenzoxy-L-lysyl-L-proline benzylester.

All the thus obtained oily material was dissolved in methanol (20 ml) and 1N sodium hydroxide (20 ml) was added thereto under cooling. The mixture was stirred for 3 hours, and the aqueous solution was adjusted to pH 7 by addition of 1N hydrochloric acid. The methanol was distilled off under reduced pressure. The aqueous solution was adjusted to pH 2 by addition of 1N hydrochloric acid, and the desired product was extracted with ethyl acetate. The ethyl acetate phase was washed with water and dried with anhydrous sodium sulfate. The ethyl acetate was distilled off under reduced pressure to obtain an oily t-butyloxycarbonyl-ε-carbobenzoxy-L-lysyl-L-proline.

All the above oily material was dissolved in DMF (20 ml), and L-proline benzylester hydrochloride (4 g, 16.7 m mole), HOBt (2.2 g, 16.7 m mole) and WSC (3 ml, 16.7 m mole) were added thereto while cooling to -15° C.

The mixture was stirred overnight and excess of ethyl acetate was added thereto. The ethyl acetate solution was washed with 1N hydrochloric acid, 5% sodium bicarbonate and water in order, and dried with anhydrous sodium sulfate. The ethyl acetate was distilled off under reduced pressure to obtain an oily t-butyloxycarbonyl-ε-carbobenzoxy-L-lysyl-L-prolyl-L-proline benzylester (11 g).

B. Diethylphosphoryl-L-lysyl-L-prolyl-L-proline L-arginine salt

Trifluoroacetic acid (50 ml) was added to the oily t-butyloxycarbonyl-ε-carbobenzoxy-L-lysyl-L-prolyl-L-proline benzylester (11 g) under cooling and the mixture was stirred for 40 minutes. Trifluoroacetic acid was distilled off under reduced pressure and the residue was dissolved in DMF (30 ml), and was neutralized by addition of triethylamine, and then diethylphosphorylchloride carbon tetrachloride solution (25 m mole) was added thereto while keeping the temperature to not more than 5° C. and keeping usually pH value to 8-9. The mixture was stirred for 4 hours at room temperature. The solvent was distilled off under reduced pressure and the residue was dissolved in ethyl acetate, and washed with 1N hydrochloric acid, 5% sodium bicarbonate and water in order. The solution was dried with anhydrous sodium sulfate, and the solvent was distilled off under reduced pressure to obtain an oily diethylphosphoryl-ε-carbobenzoxy-L-lysyl-L-prolyl-L-proline benzylester (9.5 g). The oily material (9.5 g) was dissolved in methanol (100 ml) and palladium-carbon (5 g) was added thereto, and thereafter catalytic reductive reaction was carried out for 4 hours. The catalyst was removed by filtration and the solvent was distilled off under reduced pressure to obtain an oily diethylphosphoryl-L-lysyl-L-prolyl-L-proline. The oily material was dissolved in ethanol and L-arginine (2.3 g) water (4 ml) solution was added thereto. The solvent was distilled off under reduced pressure and water was removed by azeotropic distillation with toluene. To the residue ethanol was added and thus produced insoluble material was removed by filtration. By adding ethyl acetate to the filtrate, diethylphosphoryl-L-lysyl-L-prolyl-L-proline L-arginine salt (3.5 g) was obtained.

After hydrolysis of the product with 6N hydrochloric acid, amino acids were analyzed. Lysine:Proline:Arginine=0.99:1.57:1.00

EXAMPLE 24

Dibenzylphosphoryl-L-alanyl-L-prolyl-L-proline L-lysine salt:

L-lysine (701 mg, 4.8 m mole) was added to the oily dibenzylphosphoryl-L-alanyl-L-prolyl-L-proline (2.72 g, 5 m mole), and water (40 ml) was added thereto to dissolve the solid substances, and the solution was freeze-dried to obtain an amorphous powder of dibenzylphosphoryl-L-alanyl-L-prolyl-L-proline L-lysine salt (3.4 g).

Elementary analysis: Found C 53.94%, H 7.50%, N 9.58% Calculation C 53.94%, H 7.27%, N 9.53% as C₃₃ H₄₈ N₅ O₉ P.2.5H₂ O

EXAMPLE 25

Diethylphosphoryl-L-alanyl-L-prolyl-L-proline L-arginine salt:

A. Diethylphosphorylchloride

Phosphorous acid diethylester (7 g, 50 m mole) was dissolved in carbon tetrachloride solution (50 m mole, 70 ml), and to the solution sulfurylchloride (6.8 g) carbon tetrachloride solution (25 ml) was added dropwise under the temperature not more than 5° C. with passing of nitrogen gas. After the addition, the mixture was stirred for 1.5 hours with passing of nitrogen gas. The reaction was traced by thin layer chromatography (developing solvent, chloroform:ethanol:ethyl acetate=5:2:5; Colour former, Iodine). It was confirmed that the starting materials were did not remaind in the reaction solution, and thus produced drethylphosphorylchloride was used for next step reaction without further purification.

B. Diethylphosphoryl-L-alanyl-L-proline benzylester

Trifluoroacetic acid (50 ml) was added to t-butyloxycarbonyl-L-alanyl-L-proline benzylester (11.3 g, 30 m mole) under cooling and the mixture was stirred for 40 minutes. Trifluoroacetic acid was distilled off under reduced pressure and the residue was dissolved in DMF and neutralized by addition of triethylamine (8 ml) under cooling to -5° C. Next, diethylphosphorylchroride carbon tetrachloride solution (40 m mole) was added dropwisely thereto while keeping the temperature to not more than 5° C., and keeping pH value to 8-9 and the mixture was stirred overnight at room temperature. The solvent was distilled off under reduced pressure, and the residue was dissolved in ethyl acetate. The solution was washed with 1N hydrochloric acid, 5% sodium bicarbonate and water in order, and dried with anhydrous sodium sulfate. The solvent was distilled off under reduced pressure to obtain an oily diethylphosphoryl-L-alanyl-L-proline benzylester (9.2 g).

C. Diethylphosphoryl-L-alanyl-L-prolyl-L-proline L-arginine salt:

The oily diethylphosphoryl-L-alanyl-L-proline benzylester (8.5 g, 20.6 m mole) was dissolved in methanol and palladium-carbon (2 g) was added thereto. The reductive reaction was carried out for 3 hours. The catalyst was removed by filtration and the solvent was distilled off under reduced pressure to obtain an oily diethylphosphoryl-L-alanyl-L-proline.

All the thus obtained oily material and L-proline benzylester hydrochloride (5.3 g, 22 m mole) were dissolved in methylene dichloride (50 ml) and WSC (4 ml, 22 m mole) was added thereto while cooling to -15° C. The mixture was stirred for 3 hours under cooling and overnight at room temperature, and the solvent was distilled off under reduced pressure. The residue was dissolved in ethyl acetate, and washed with 1N hydrochloric acid, 5% sodium bicarbonate and water in order and dried with anhydrous sodium sulfate. The solvent was distilled off under reduced pressure to obtain an oily diethylphosphoryl-L-alanyl-L-prolyl-L-proline benzylester (9.7 g).

All the thus obtained oily material was dissolved in methanol and palladium-carbon (2 g) was added thereto. The reductive reaction was carried out for 3 hours. The catalyst was separated by filtration and the solvent was distilled off under reduced pressure. The residue was purified by silica gel chromatography (solvent, chloroform:methanol:acetic acid=95:5:3) to obtain an oily diethylphosphoryl-L-alanyl-L-prolyl-L-proline (3.1 g). The oily material was dissolved in ethanol and L-arginine (1.3 g) water (2 ml) solution was added thereto. The solvent was distilled off under reduced pressure and water was removed by azeotropic distillation with toluene. Ethanol was added to the residue and thus obtained insoluble material was separated by filtration. By adding ethyl acetate to the filtrate, diethylphosphoryl-L-alanyl-L-prolyl-L-proline arginine salt (1.3 g) was obtained. Melting point: 98° to 102° C., Specific rotatory power [α]_(D) ²⁰ =-77.9° C. (C=0.65 ethanol).

After hydrolysis of the product with 6N hydrochloric acid, amino acids were analyzed. Alanine:Proline:Arginine=1.00:2.04:0.80.

EXAMPLE 26

N-(O-benzyl-P-phenethylphosphonyl)-L-alanyl-L-prolyl-L-proline benzylester.

A. L-alanyl-L-prolyl-L-proline benzylester hydrochloride

N-t-butyloxycarbonyl-L-alanyl-L-prolyl-L-proline benzylester (6.0 g, 12.7 m mole) as produced in the same manner as in Example 1, was dissolved in 4.8N hydrogene chloride dioxane solution, and the mixture was stirred for 1 hour at room temperature. The solvent was distilled off under reduced pressure and by adding ethyl ether to the residue for crystallization to obtain L-alanyl-L-prolyl-L-proline benzylester hydrochloride (5.18 g, yield: 99.7%).

B. Dibenzylphenethylphosphonate

Dibenzyl hydrogene phosphite (12.3 g, 50 m mole) was dissolved in DMF (80 ml), and sodium hydride (60% in oil, 2.2 g) was added to the mixture at an atmosphere of nitrogen while cooling to -15° C. and stirring. The thus obtained mixture was stirred for 1.5 hours at the temperature not more than 0° C. Phenethyl bromide (9.25 g, 50 m mole) in DMF (10 ml) solution was added dropwise to the above solution at an atmosphere of nitrogen at the temperature not more than 0° C. The thus obtained mixture was further reacted overnight at room temperature. DMF was distilled off under reduced pressure and the residue was dissolved in ethyl ether, and the resulting solution was washed with water, and dried with anhydrous sodium sulfate. The solvent was distilled off under reduced pressure to obtain an oily dibenzylphenethylphosphonate. The product was crystallized by standing it at room temperature. The product was recrystallized with ethyl ether to obtain dibenzylphenethylphosphonate (6.5 g, yield: 35.5%) having melting point: 48° to 49° C.

B. Monobenzylphenethylphosphochloridate

Dibenzylphenethylphosphonate (3.19 g, 8.7 m mole) was dissolved in carbon tetrachloride (5 ml) and phosphorus pentachloride (1.91 g, 9.2 m mole) was added thereto under cooling in the ice bath, and the mixture was stirred for 30 minutes. The mixture was heated to 70° C. in the rate of 10° C. per 15 minutes and stirred for 30 minutes at 70° C. The reaction was completed by standing it for 2 hours at 70° C./1mHg in the rotating evaporator and then the solvent, phosphorus oxychloride and benzyl chloride were distilled off. It was confirmed that the starting materials did not remain by tracement of silica gel thin layer chromatography (developing solvent, benzene:ethyl acetate=1:1, Confirmation of Spot: UV lamp), and thus produced monobenzylphosphochloridate was used for the following reaction without further purification.

C. N-(O-benzyl-P-phenethylphosphonyl)-L-alanyl-L-prolyl-L-proline benzylester

L-alanyl-L-prolyl-L-proline benzylester hydrochloride (2.5 g, 6.1 m mole) was dissolved in methylene dichloride (20 ml) and triethyl amine (1.85 g, 18.3 m mole) was added thereto. Monobenzylphenethylphosphochloridate which had been produced from dibenzylphenethylphosphonate (3.19 g, 8.7 m mole) was dissolved in methylene dichloride (10 ml). This solution was added dropwise to the above solution under cooling in the ice bath and the mixture was stirred overnight at room temperature. The reaction solution was washed with water and the solvent was distilled off under reduced pressure. The residue was dissolved in ethyl acetate and the mixture was washed with 1N hydrochloric acid, water, 5% sodium bicarbonate and water in order, and dried with anhydrous sodium sulfate. The solvent was distilled off under reduced pressure to obtain a sticky residue and it was purified by column chromatography (silica gel, developing solvent; ethyl acetate:methanol=20:1) to obtain N-(O-benzyl-P-phenethylphosphonyl)-L-alanyl-L-proline benzylester (1.58 g, yield: 41.7%). The product gave a single spot with R_(f) =0.4 by thin layer chromatography (developing solvent; ethyl acetate:methanol=20:1, colour forming method: spraying of 25% hydrobromic acid and 0.1% ninhydrine and then heating).

NMR spectrum [CDCl₃, internal standard: TMS] ppm: 1.30 (2xd,3H), 1.70˜2.37 (m, 10H), 2.70˜3.10 (m, 2H), 3.30˜3.90 (m, 4H), 4.00˜4.35 (m, 1H), 4.45˜4.75 (m, 2H), 4.90˜5.33 (m, 4H), 7.20 (S, 5H), 7.33 (S, 10H).

EXAMPLE 27

N-(O-benzyl-P-phenethylphosphonyl)-L-alanyl-L-prolyl-L-proline L-arginine salt:

N-(O-benzyl-P-phenethylphosphonyl)-L-alanyl-L-prolyl-L-proline benzylester (0.88 g, 1.4 m mole) was dissolved in acetone (5 ml) and sodium hydroxide (0.18 g, 4.5 m mole) in water (4 ml) solution was added thereto under cooling in the ice bath, and the mixture was stirred for 2 hours. To the solution water (100 ml) was added and the mixture was washed with ethyl ether (50 ml). The solution was neutralized with 1N hydrochloric acid, and the desired product was extracted with ethyl ether (100 ml). The ethyl ether solution was dried with anhydrous sodium sulfate. The solvent was distilled off under reduced pressure to obtain an oily N-(O-benzyl-P-phenethylphosphonyl)-L-alanyl-L-prolyl-L-proline (0.54 g, yield: 71.6%). All the thus obtained oily material (1.0 m mole) was dissolved in ethanol (4 ml) and the solution produced by dissolving L-arginine (0.21 g, 1.0 m mole) in water (4 ml) was added thereto, and then the solvent was distilled off under reduced pressure. By adding further water to it and freeze-drying the mixture, N-(O-benzyl-P-phenethylphosphonyl)-L-alanyl-L-prolyl-L-proline L-arginine salt (0.59 g, yield: 60.2% from N-(O-benzyl-P-phenethylphosphonyl)-L-alanyl-L-prolyl-L-proline benzylester) was obtained.

A fixed amount of the product (sample) was heated for 19 hours at 110° C. in 6N hydrochloric acid. Amino acids in thus hydrolized material were analyzed. The ratio of alanine, proline and arginine was 1.00:2.08:1.05.

EXAMPLE 28

N-phenethylphosphonyl-L-alanyl-L-prolyl-L-proline 2 sodium salt:

N-(O-Benzyl-P-phenethylphosphonyl)-L-alanyl-L-prolyl-L-proline benzylester (0.67 g, 1.1 m mole) was dissolved in the mixture (24 ml) of water and methanol (1:1) and sodium bicarbonate (0.16 g, 2.2 m mole) was added thereto. Hydrogen gas was passed through the solution for 3 hours under atmospheric pressure at room temperature in the presence of 5% palladium carbon as a catalyst.

The catalyst was separated by filtration and the solvent was distilled off. By dissolving the residue in water and freeze-drying it, N-phenethylphosphonyl-L-alanyl-L-prolyl-L-proline 2 sodium salt (0.54 g, quantitative yield) was obtained.

A fixed amount of the product was heated for 19 hours at 110° C. in 6N hydrochloric acid. For the thus hydrolized material, amino acids were analyzed. The ratio of alanine and proline was 1.00:2.10.

NMR spectrum [D₂ O, internal standard: DSS] ppm: 1.25 (d, 3H), 1.50˜2.45 (m, 10H), 2.60˜3.00 (m, 2H), 3.30˜3.90 (m, 4H), 4.00˜4.56 (m, 4H), 7.30 (S, 5H)

EXAMPLE 29

N-Phenethylphosphonyl-L-alanyl-N-(2-indanyl)glycyl-L-proline 2 sodium salt:

A. N-t-Butyloxycarbonyl-glycyl-L-proline benzylester

L-proline benzylester hydrochloride (20g, 82.7 m mole) was added to chloroform (200 ml) and triethylamine (8.3 g, 82.7 m mole), N-t-butyloxycarbonyl-glycine (14.5 g, 82.7 m mole), and HOBt (11.2 g, 82.7 m mole) were added thereto under cooling. To the mixture WSC hydrochloride (15.82 g, 82.7 m mole) was added dropwise.

Further, the reaction was carried out for 2 hours under cooling with ice, and next, overnight at room temperature. The reaction solution was washed with 10% citric acid, aqueous sodium chloride, 5% sodium bicarbonate and aqueous sodium chloride in order, and dried with anhydrous sodium sulfate. The solvent was distilled enough off under reduced pressure to obtain a sticky N-t-butyloxycarbonyl-glycyl-L-proline benzylester (31.4 g, 83.5 m mole; yield: 100%). The product gave a single spot with R_(f) =0.66 by silica gel thin layer chromatography (developing solvent, benzene:ethyl acetate=1:1, Colour forming method: spraying of 25% hydrobromic acid and 0.1% ninhydrin and heating).

NMR spectrum [CDCl₃, interll standard: TMS] ppm: 1.44 (S, 9H); 1.83˜2.30 (m, 4H), 3.33˜3.72 (m, 2H), 3.90 (d, 2H), 4.44˜4.67 (m, 1H), 5.12 (S, 2H), 5.42 (br, 1H), 7.32 (S, 5H).

B. Glycyl-L-proline benzylester hydrochloride

To N-t-butyloxycarbonyl-glycyl-L-proline benzylester (25.7 g, 68.2 m mole) 4N hydrogen chloride dioxane solution (170 ml) was added and the solid material was dissolved. The mixture was stirred for 1 hour at room temperature. The solvent was evaporated under reduced pressure to obtain a half-solid glycyl-L-proline benzylester hydrochloride (20.2 g, 64.8 m mole, yield: 95%).

The product had a hygroscopicity and gave a single spot with R_(f) =0.27 by silica gel thin layer chromatography (developing solvent, chloroform:methanol:triethylamine=40:2:1, colour forming method: spraying of 0.1% ninhydrin, and heating).

NMR spectrum [CDCl₃, internal standard: TMS] ppm: 1.76˜2.26 (m, 4H), 3.39˜3.80 (m, 2H), 3.98˜4.31 (m, 2H), 4.46˜4.73 (m, 1H), 5.06 (S, 2H), 7.26 (S, 5H), 8.34 (br, 3H)

C. N-(2-indanyl) glycyl-L-proline benzylester

Glycyl-L-proline benzylester hydrochloride (20.0 g, 64.1 m mole) was dissolved in dried ethanol (150 ml) and indan-2-on (4.23 g, 32.1 m mole), and Molecular Sieves (3A) (5 g) were added thereto. Sodium cyanoborohydride (2.01 g, 32.1 m mole) was gradually added to the above solution while cooling with ice, and stirring. The mixture was stirred for 1 hour under cooling with ice and then stirred for 2 hours at room temperature. A little insoluble material was separated by filtration. The ethanol was removed by distillation under reduced pressure from the reaction solution. To the residue water (150 ml) and ethyl ether (200 ml) were added and the solution was adjusted to pH 1.5 with 6N hydrochloric acid while stirring to dissolve it. The ethylether layer was removed and the remaining water layer was washed with ethyl ether (200 ml). Next, to the water layer ethyl ether (400 ml) was added and the solution was adjusted to pH 8.0 with sodium bicarbonate aqueous solution. The desired product was extracted with ethyl ether and the ethyl ether layer was separated. Further, the desired product was extracted by the addition of ethyl ether (200 ml) to the water layer and the thus extracted ethyl ether solution was separated. Such extracted ethyl ether layers were combined together, and washed with the aqueous solution saturated with sodium chloride (100 ml). The ethyl ether layer was dried with anhydrous sodium sulfate, and concentrated under reduced pressure to obtain a crystal. The crystal was washed with n-hexane, obtained on the filter paper and dried to yield a white crystal of N-(2-indanyl)glycyl-L-proline benzylester (8.02 g, 21.2 m mole, yield: 33%). Melting point: 96°-98° C.

The product gave a single spot with R_(f) =0.77 and R_(f) =0.42, respectively by silica gel thin layer chromatography (Developing solvent, n-butanol:acetic acid:water=4:2:2; and chloroform:methanol:triethylamine=40:2:1, Colour forming method: spraying of 0.1% ninhydrine, and heating).

NMR spectrum [CDCl₃, internal standard: TMS] ppm: 1.82˜2.34 (m, 4H), 2.70˜3.82 (m, 9H), 4.44˜4.72 (m, 1H), 5.14 (S, 2H), 7.13 (S, 4H), 7.32 (S, 5H).

D. N-t-Butyloxycarbonyl-L-alanyl-N-(2-indanyl) glycyl-L-proline benzylester

N-(2-indanyl) glycyl-L-proline benzylester (6.0 g, 15.9 m mole), N-t-butyloxycarbonyl-L-alanine (3.0 g, 15.9 m mole) and HOBt (2.1 g, 15.9 m mole) were dissolved in chloroform (60 ml) and the mixture was stirred under cooling with ice, and WSC hydrochloride (3.03 g, 15.9 m mole) was added dropwise thereto. The reaction solution was stood for 4 days in a refrigerator. Next, to the reaction solution chloroform (100 ml) was added and the mixture was washed with 10% citric acid, aqueous sodium chloride, 5% sodium bicarbonate and aqueous sodium chloride in order, and dried with anhydrous sodium sulfate. The solvent was distilled off under reduced pressure. The syrupy residue was dissolved in ethyl ether (300 ml), and water (200 ml) was added thereto. The pH value was adjusted to 1.5 with 6N hydrochloric acid and the mixture was washed. The ethyl ether layer was separated and then dried with anhydrous sodium sulfate.

Ethyl ether was removed by distillation under reduced pressure to obtain the crystal. The crystalls were washed with n-hexane and recrystalized with ethyl acetate - isopropyl ether-n-hexan to obtain a white crystal of N-t-butyloxycarbonyl-L-alanyl-N-(2-indanyl) glycyl-L-proline benzylester (3.02 g, 5.5 m mole, yield: 35%). Melting point: 135°-136° C.

The product gave a single spot with R_(f) =0.63 by silica gel thin layer chromatography (developing solvent; benzene:ethyl acetate=1:1, Colour forming method: spraying of 25% hydrobromic acid and 0.1% ninhydrin, and heating).

Unreacted starting materials of N-(2-indanyl) glycyl-L-proline benzylester (2.95 g) was recovered from the aqueous solution of pH 1.5 produced by washing the above mentioned ethyl ether phase.

NMR spectrum [CDCl₃, internal standard: TMS] ppm: 1.25 (d, 3H), 1.40 (S, 9H), 1.65˜2.25 (m, 4H), 2.55˜3.85 (m, 7H), 3.90˜5.00 (m, 4H), 5.05 (S, 2H), 5.40 (d, 1H), 7.10 (S, 4H), 7.23 (S, 5H).

E. L-Alanyl-N-(2-indanyl) glycyl-L-proline benzylester hydrochloride

N-t-butyloxycarbonyl-L-alanyl-N-(2-indanyl) glycyl-L-proline benzylester (2.72 g, 4.95 m mole) was dissolved in 4N hydrogen chloride dioxane solution (50 ml) and the mixture was stirred for 1 hour at room temperature. The solvent was distilled off under reduced pressure from the solution, and thereby a sticky residue was obtained. The residue was made into a crystalline form by the addition of ethyl ether, and the crystal was obtained on the filter paper, and immediately dried in the desiccator to obtain a white powder of L-alanyl-N-(2-indanyl) glycyl-L-proline benzylester hydrochloride (2.31 g, 4.76 m mole, yield: 96%). This product had a hygrospic nature, and gave a single spot with R_(f) =0.42 by silica gel thin layer chromatography (developing solvent, chloroform:methanol:triethylamine=40:2:1, Colour forming method: spraying of 0.1% ninhydrin and heating).

NMR spectrum [CDCl₃, internal standard: TMS] ppm: 1.20 (2xd, 3H), 1.37˜2.45 (m, 4H), 2.50˜3.90 (m, 7H), 3.93˜5.00 (m, 4H), 5.10 (S, 2H), 7.15 (S, 4H), 7.30 (S, 5H), 8.40 (br, 3H).

F. N-(O-benzyl-P-phenethylphosphonyl)-L-alanyl-N-(2-indanyl) glycyl-L-proline benzylester

L-alanyl-N-(2-indanyl) glycyl-L-proline benzylester hydrochloride (1.5 g, 3.09 m mole) was dissolved in methylene dichloride (15 ml), and triethylamine (1.03 g, 10.2 m mole) was added thereto. Monobenzylphenethylphosphochloridate produced from dibenzylphenethylphosphonate (1.61 g, 4.4 m mole) was dissolved in methylene dichloride (10 ml). This solution was dropwise added to the above solution under cooling with ice bath and further stirred for 2 hours at room temperature. The reaction solution was washed with water, and the solvent was distilled off under reduced pressure. The residue was dissolved in ethyl acetate, and the resulting solution was washed with 1N hydrochloric acid, water, 5% sodium bicarbonate and water in order, and dried with anhydrous sodium sulfate. The solvent was distilled off under reduced pressure to obtain a sticky residue. It was purified by column chromatography (silica gel, developing solvent; ethyl acetate, methanol=20:1) to obtain N-(O-benzyl-P-phenethylphosphonyl)-L-alanyl-N-(2-indanyl) glycyl-L-proline benzylester (1.03 g, 1.46 m mole; yield: 47%).

This product gave a single spot with R_(f) =0.32, by thin layer chromatography (developing solvent, benzene:ethyl acetate=1:1, Colour forming method: spraying of 25% hydrobromic acid and 0.1% ninhydrin and heating).

NMR spectrum [CDCl₃, internal standard: TMS] ppm: 1.20˜1.55 (m, 3H), 1.65˜2.30 (m, 6H), 2.50˜4.70 (m, 13H), 4.80˜5.17 (m, 4H), 7.10 (S, 4H), 7.15 (S, 5H), 7.25 (S, 5H), 7.30 (S, 5H).

G. N-Phenethylphosphonyl-L-alanyl-N-(2-indanyl) glycyl-L-proline 2 sodium salt

N-(O-Benzyl-P-phenethylphosphonyl)-L-alanyl-N-(2-indanyl) glycyl-L-proline benzylester (0.74 g, 1.0 m mole) was dissolved in a mixture (30 ml) of water and ethanol (2:3) and sodium bicarbonate (0.176 g, 2.0 m mole) was added thereto. Hydrogen gas was passed through the solution for 3 hours at room temperature and atmospheric pressure in the presence of 5% palladium carbon as a catalyst. The catalyst was separated by filtration and the solvent was distilled off under reduced pressure. By dissolving the residue in water and freeze-drying it, N-phenethylphosphonyl-L-alanyl-N-(2-indanyl) glycyl-L-proline 2 sodium salt (0.57 g, yield: 95.4%) was obtained.

NMR spectrum [D₂ O, internal standard: DSS] ppm: 1.05˜1.52 (m, 3H), 1.55˜2.35 (m, 6H), 2.40˜about 4.30 (m, 13H: peak masked partially with water), 7.28 (S, 4H), 7.35 (S, 5H).

EXAMPLE 30

N-(O-Benzyl-P-phenethylphosphonyl)-L-alanyl-N-(2-indanyl) glycyl-L-proline L-arginine salt:

A. N-t-Butyloxycarbonyl-glycyl-L-proline methylester L-proline methylester hydrochloride (13.6 g, 82.7 m mole) was suspended in chloroform (200 ml) and to the suspended solution triethylamine (8.3 g, 82.7 m mole) was added while cooling. To the solution, N-t-butyloxycarbonyl glycine (14.5 g, 82.7 m mole) was added and WSC hydrochloride (15.82 g, 82.7 m mole) was gradually added thereto while cooling with ice. The reaction was carried out for 2 hours while cooling with ice, and next overnight at room temperature. The reaction solution was washed with 10% citric acid, aqueous sodium chloride, 5% sodium bicarbonate and aqueous sodium chloride in order, and dried with anhydrous sodium sulfate. The solvent was distilled off under reduced pressure to obtain a syrupy N-t-butyloxycarbonylglycyl-L-proline methylester (22.5 g, 78.5 m mole, yield: 95%). This product gave a single spot with R_(f) =0.48 by silica gel thin layer chromatography (developing solvent, benzene:ethyl acetate=1:1, colour forming method: spraying of 25% hydrobromic acid and 0.1% ninhydrin and heating).

NMR spectrum [CDCl₃, internal standard: TMS] ppm: 1.44 (S, 9H), 1.92˜2.32 (m, 4H), 3.45˜3.75 (m, 2H), 3.7 (S, 3H), 3.92 (d, 2H), 4.40˜4.55 (m, 1H), 5.54 (br, 1H).

B. Glycyl-L-proline methylester hydrochloride N-t-Butyloxycarbonyl-glycyl-L-proline methylester (21.3 g, 74.4 m mole) and-4N hydrogen chloride dioxan solution (150 ml) were mixed, and shaked to dissolve the solid material. It was stirred for 1 hour at room temperature. The solvent was well-distilled off under reduced pressure to obtain the solid glycyl-L-proline methylester hydrochloride (16.4 g, 73.9 m mole, yield: 99%). The product was very hygroscopic and gave a single spot with R_(f) =0.45 by silica gel thin layer chromatography (developing solvent, n-butanol:acetic acid:water=4:2:2, colour forming method: spraying of 0.1% ninhydrin and heating).

NMR spectrum [CDCl₃, internal standard: TMS] ppm: 1.84˜2.40 (m, 4H), 3.48˜4.27 (m, 4H), 3.67 (S, 3H), 4.43˜4.65 (m, 1H), 8.22 (br, 3H).

C. N-(2-indanyl) glycyl-L-proline methylester hydrochloride

Glycyl-L-proline methylester hydrochloride (16.0 g, 72.1 m mole) was dissolved in methanol (150 ml), and Indan-2-on (4.75 g, 36.0 m mole) and triethylamine (1.8 g, 18.0 m mole) were added thereto. Sodium syanoborohydride (2.27 g, 36.0 m mole) was gradually added thereto while cooling with ice and stirring. The mixture was stirred for 1 hour while cooling with ice and next for 5 hours at room temperature. A little insoluble material was separated by filtration, and the filtrate was concentrated under reduced pressure. To the residue, water (100 ml) and ethyl ether (100 ml) were added, and the solution was adjusted to pH 1.5 with 6N hydrochloric acid while stirring to dissolve it. The ethyl ether layer was removed, and the remaining water layer was washed with ethyl ether (100 ml). The desired product was extracted from the water layer with chloroform (100 ml×3 times). The thus obtained chloroform as extracted was concentrated under reduced pressure. To thus produced tar-like residue ethyl ether and 5% sodium bicarbonate (200 ml) were added and the mixture was stirred to dissolve the solid matter. The aqueous layer was removed and the ethyl ether layer was washed with aqueous solution saturated with sodium chloride. To the ethyl ether layer water (150 ml) was added, and the solution was adjusted to pH 1.5 with 6N hydrochloric acid while stirring and ethyl ether was removed. The water layer was washed with ethyl ether (100 ml) and to the water layer chloroform (200 ml) was added. The solution was adjusted to pH 8.0 with sodium bicarbonate while stirring. The aqueous layer was removed, and chloroform layer was dried with anhydrous sodium sulfate, and the sodium sulfate was sepa rated by filtration. Separately, anhydrous hydrogen chloride gas was brown into the chloroform, and thus obtained hydrogen chloride chloroform solution was added to the above mentioned chloroform layer. After checking the solution acidic enough, the chloroform was removed off under reduced pressure, to yield N-(2-indanyl) glycyl-L-proline methylester hydrochloride (4.50 g, 13.3 m mole, yield: 37%).

This product gave a single spot respectively in the R_(f) =0.73 and R_(f) =0.58, by silica gel thin layer chromatography (developing solvent, n-butanol:acetic acid:water=4:2:2, and chloroform:methanol:triethylamine=40:2:1, colour forming method: spraying of 0.1% ninhidrin and heating).

NMR spectrum [CDCl₃, internal standard: TMS] ppm: 1.81˜2.38 (m, 4H), 3.26 (d, 4H), 3.40˜3.93 (m, 4H), 3.66 (S, 3H), 4.03 (t, 1H), 4.40˜4.66 (m, 1H), 5.29 (S, 2H), 7.16 (S, 4H).

D. N-t-Butyloxycarbonyl-L-alanyl-N-(2-indanyl) glycyl-L-proline methylester

N-(2-indanyl) glycyl-L-proline methylester hydrochloride (4.33 g, 12.8 m mole), N-t-butyloxycarbonyl-L-alanine (2.42 g, 12.8 m mole) and HOBt (1.73 g, 12.8 m mole) were dissolved in chloroform (50 ml), and triethylamine (1.29 g, 12.8 m mole) was added thereto while cooling to -15° C. and then WSC hydrochloride (2.45 g, 12.8 m mole) was gradually added thereto under cooling with ice. Further, the reaction was carried out for 2 hours under cooling with ice and next overnight at room temperature. The reaction solution was washed with 10% citric acid, aqueous sodium chloride, 5% sodium bicarbonate, and aqueous sodium chloride in order, and dried with anhydrous sodium sulfate. The solution was concentrated under reduced pressure to obtain the syrupy material (3.88 g). It was further purified by column chromatography (silica gel, developing solvent, chloroform and next ethyl acetate) to obtain N-t-butyloxycarbonyl-L-alanyl-N-(2-indanyl) glycyl-L-proline methylester (2.42 g, yield: 64%). The product gave a single spot with R_(f) =0.4 by thin layer chromatography (developing solvent, ethyl acetate:benzene=1:1, colour forming method: spraying of 25% hydrobromic acid and 0.1% ninhydrin and heating).

NMR spectrum [CDCl₃, internal standard: TMS] ppm: 1.30 (d, 3H), 1.45 (S, 9H), 1.75˜2.40 (m, 4H), 2.70˜3.85 (m, 7H), 3.70 (S, 3H), 3.90˜5.10 (m, 4H), 5.45 (d, 1H), 7.20 (S, 4H).

N-(2-indanyl) glycyl-L-proline methylester hydrochloride (2.08 g) was recovered from the 10% citric acid solution which was used for washing the above mentioned reaction mixture.

E. L-Alanyl-N-(2-indanyl) glycyl-L-proline methylester hydrochloride

N-t-Butyloxycarbonyl-L-alanyl-N-(2-indanyl) glycyl-L-proline methylester (2.24 g, 4.7 m mole) was dissolved in 4.8N hydrogen chloride dioxane solution and stirred for 1 hour at room temperature. The solvent was distilled off under reduced pressure and diethyl ether was added to the residue for crystallization to obtain L-alanyl-N-(2-indanyl) glycyl-L-prolyl methylester hydrochloride (1.63 g, yield: 84.1%).

NMR spectrum [D₂ O, internal standard: DSS] ppm: 1.03˜1.37 (m, 3H), 1.60 (br, 3H), 1.75˜2.55 (m, 4H), 2.55˜3.60 (m, 7H), 3.75 (S, 3H), 3.85˜about 4.5 (m, 4H, peak partially masked with water), 7.30 (S, 4H).

F. N-(O-Benzyl-P-phenethylphosphonyl)-L-alanyl-N-(2-indanyl) glycyl-L-proline methylester

L-Alanyl-N-(2-indanyl) glycyl-L-proline methylester hydrochloride (1.0 g, 2.4 m mole) was dissolved in methylene dichloride (7 ml) and triethylamine (0.77 g, 7.6 m mole) was added thereto. Monobenzylphenethylphosphochloridate produced from dibenzylphenethylphosphonate (1.33 g, 3.6 m mole) was dissolved in methylene dichloride (3 ml). This solution was dropwise added to the above solution while cooling in the ice bath. The mixture was stirred overnight at room temperature. To the reaction solution methylene dichloride (50 ml) was added, and thus obtained mixture was washed with 1N hydrochloric acid, water, 5% sodium bicarbonate and water in order and dried with anhydrous sodium sulfate. The solvent was distilled off under reduced pressure to obtain a sticky material (1.83 g). It was purified by column chromatography (silica gel, developing solvent, ethyl acetate, and next, ethyl aqetate:methanol=20:1) to obtain N-(O-benzyl-P-phenethylphosphonyl)-L-alanyl-N-(2-indanyl) glycyl-L-proline methylester (0.65 g, yield: 42.2%). The product gave a single spot with R_(f) =0.25 by thin layer chromatography (developing solvent, ethyl acetate, colour forming method: spraying of 25% hydrobromic acid and 0.1% ninhydrin and heating).

NMR spectrum [CDCl₃, interal standard: TMS] ppm: 1.35 (m, 3H), 1.70˜2.50 (m, 6H), 2.60˜3.85 (m, 9H), 3.65 (S, 3H), 3.85˜4.70 (m, 4H), 4.97 (d, 2H), 5.47 (br, 1H), 7.20 (S, 9H), 7.30 (S, 5H).

G. N-(O-Benzyl-P-phenethylphosphonyl)-L-alanyl-N-(2-indanyl) glycyl-L-proline

N-(O-Benzyl-P-phenethylphosphonyl)-L-alanyl-N-(2-indanyl) glycyl-L-proline methylester (0.58 g, 0.9 m mole) was dissolved in acetone (3 ml), and sodium hydroxide (0.11 g, 2.8 m mole) in water (3 ml) solution was added thereto while cooling in the ice bath. The mixture was stirred for 1 hour. To the solution water (100 ml) was added and the mixture was washed with ethyl ether (50 ml). The mixture was neutralized with 1N hydrochloric acid and the desired product was extracted with ethyl acetate. The ethyl acetate layer was dried with anhydrous sodium sulfate and the solvent was distilled off under reduced pressure to obtain a sticky N-(O-benzyl-P-phenethylphosphonyl)-L-alanyl-N-(2-indanyl) glycl-L-proline (0.53 g, yield: 93.5%).

NMR spectrum [CDCl₃, internal standard: TMS] ppm: 1.20˜1.50 (m, 3H), 1.60˜2.32 (m, 4H), 2.40˜4.75 (m, 11H), 4.90 (d, 2H), 7.03 and 7.10 (2xS, 9H), 7.23 (S, 5H).

H. N-(O-Benzyl-P-phenethylphosphonyl)-L-alanyl-N-(2-indanyl) glycyl-L-proline L-arginine salt

N-(O-benzyl-P-phenethylphosphonyl)-L-alanyl-N-(2-indanyl) glycyl-L-proline (0.53 g, 0.9 m mole) was dissolved in ethanol (4 ml) and the aqueous solution produced by dissolving L-arginine (0.17 g, 0.9 m mole) in water (4 ml) was added thereto. The solvent was distilled off under reduced pressure and water was added thereto. By freeze-drying it, N-(O-benzyl-P-phenethylphosphonyl)-L-alanyl-N-(2-indanyl) glycyl-L-proline L-arginine salt (0.64 g, yield: 94.5%) was obtained. The product gave a single spot with R_(f) =0.25 for peptide in the free form and a single spot with R_(f) =0 for arginine, respectively, by thin layer chromatography (developing solvent, acetonitrile:acetic acid=30:1, Colour forming method: spraying of 25% hydrobromic acid and 0.1% ninhydrine, and heating).

EXAMPLE 31

Dibenzylphosphoryl-L-alanyl-N-(2-indanyl)-glycyl-L-proline L-arginine salt:

A. Dibenzylphosphoryl-L-alanyl-N-(2-indanyl)-glycyl-L-proline methylester

L-alanyl-N-(2-indanyl) glycyl-L-proline methylester hydrochloride (1.44 g, 3.5 m mole) was dissolved in chloroform (20 ml), and triethylamine (1.1 g, 10.9 m mole) was added thereto. Dibenzylphosphorylchloride which had been produced from dibenzyl hydrogenphosphite (0.86 g, 3.5 m mole) in the same manner as described in Example 1 was dissolved in carbon tetrachloride (10 ml). This solution was dropwise added to the above produced solution in the ice bath. The mixture was stirred overnight at room temperature. Thus precipitated triethyl amine hydrochloride was removed by filtration and the solvent was distilled off under reduced pressure. The residue was dissolved in ethyl acetate, washed with 1N hydrochloric acid, water, 5% sodium bicarbonate and water in order, and dried with anhydrous sodium sulfate. The solvent was distilled off under reduced pressure to obtain a candy-like residue (1.64 g). It was purified by column chromatography (silica gel, developing solvent; ethyl acetate:methanol=80:1) to obtain dibenzylphosphoryl-L-alanyl-N-(2-indanyl)-glycyl-L-proline methylester (1.08 g, 48.5%). This product gave a single spot with R_(f) =0.66 by thin layer chromatography (Developing solvent, ethyl acetate:methanol=80:1, Colour forming method: spraying of 25% hydrobromic acid and 0.1% ninhydrine, and heating).

NMR spectrum [CDCl₃, internal standard: TMS] ppm: 1.10˜1.50 (m, 3H), 1.65˜2.25 (m, 4H), 2.55˜3.82 (m, 7H), 3.65 (S, 3H), 3.85˜4.80 (m, 4H), 5.00 (d, 4H), 5.45 (br, 1H), 7.16 (S, 4H), 7.35 (S, 10H).

C. Dibenzylphosphoryl-L-alanyl-N-(2-indanyl)-glycyl-L-proline

Dibenzylphosphory-L-alanyl-N-(2-indanyl) glycyl-L-proline methylester (0.79 g, 1.2 m mole) was dissolved in acetone (5 ml), and sodium hydroxide (0.15 g, 3.8 m mole) in water (4 ml) solution was added thereto while cooling in the ice bath. The mixture was stirred for 1 hour. To the solution water (100 ml) was added, and thereafter it was washed with ethyl ether (50 ml). It was neutralized with 1N hydrochloric acid, and therefrom the desired product was extracted with ethyl acetate. Thus extracted solution was dried with anhydrous sodium sulfate, and the solvent was distilled off under reduced pressure to obtain an oily dibenzylphosphoryl-L-alanyl-N-(2-indanyl) glycyl-L-proline (0.77 g, yield: 99.7%).

NMR spectrum [CDCl₃, internal standard: TMS] ppm: 1.10˜1.55 (m, 3H), 1.65˜2.30 (m, 4H), 2.40˜4.80 (m, 11 H), 5.00 (d, 4H), 7.15 (S, 4H), 7.33 (S, 10H).

D. Dibenzylphosphoryl-L-alanyl-N-(2-indanyl) glycyl-L-proline L-arginine salt

Dibenzylphosphoryl-L-alanyl-N-(2-indanyl)-glycyl-L-proline (0.77 g, 1.2 m mole) was dissolved in ethanol (4 ml), and the aqueous solution produced by dissolving L-arginine (0.25 g, 1.2 m mole) in water (4 ml) was added thereto. The solvent was distilled off under reduced pressure, and then water was added thereto. This water solution was freeze-dried to obtain dibenzylphosphoryl-L-alanyl-N-(2-indanyl) glycyl-L-proline L-arginine salt (0.84 g, yield: 85.2%). This product gave a single spot in R_(f) =0.2 for peptide in free form, and R_(f) =0 for arginine, respectively, by thin layer chromatography (developing solvent, acetonitrile:acetic acid=30:1, Colour forming method: spraying of 25% hydrobromic acid and 0.1% ninhydrine, and heating).

EXAMPLE 32

Dibenzylphosphoryl-L-alanyl-L-prolyl-L-Proline potassium salt:

To the oily dibenzylphosphoryl-L-alanyl-L-prolyl-L-proline (2.72 g, 5 m mole) 0.1 N potassium hydroxide (50 ml) was added and the thus produced solution was freeze-dried to obtain an amorphous powder of dibenzylphosphoryl-L-alanyl-L-prolyl-L-proline potassium salt (2.8 g).

Elementary analysis: Found C 51.58%, H 6.41%, N 6.70% Calculation C 51.60%, H 6.13%, N 6.69% as C₂₇ H₃₃ N₃ O₇ P.K.2.6H₂ O.

EXAMPLE 33

Dibenzylphosphoryl-L-methionyl-L-prolyl-L-proline L-arginine salt:

A. Dibenzylphosphoryl-L-methionyl-L-prolyl-L-proline methylester

L-methionyl-L-prolyl-L-proline methylester hydrochloride (2.0 g, 5.1 m mole) was dissolved in methylene chloride (20 ml), and triethyl amine (1.54 g, 15.2 m mole) was added thereto. The solution was cooled in the coolant of -30° C., and to the cooled solution, the carbon tetrachloride solution (10 ml) of dibenzylphosphorylchloride produced from dibenzylhydrogen phosphite (1.41 g, 5.1 m mole) was added dropwise, and the mixture was further stirred overnight at room temperature. To the reaction solution methylene chloride (100 ml) was added, and the mixture was washed with 1N hydrochloric acid, water, 5% sodium bicarbonate and water in order. The mixture was dried with anhydrous sodium sulfate, and the solvent was evaporated under reduced pressure to obtain a candy-like residue (3.23 g). It was purified by a column chromatography (silica gel, developing solvent; ethyl acetate:methanol=20:1) to obtain dibenzylphosphoryl-L-methionyl-L-prolyl-L-proline methylester (1.87 g, yield: 59.6%). It gave a single spot with R_(f) =0.1 by a thin layer chromatography (developing solvent, ethyl acetate:methanol=20:1, Colour forming method, spraying of 25% hydrobromic acid and 0.1% ninhydrin, and heating). NMR spectrum [CDCl₃, internal standard: TMS] ppm: 1.55˜2.30 (m, 10H), 2.03 (S, 3H), 2.60 (t, 2H), 3.30˜3.85 (m, 4H), 3.65 (S, 3H), 3.90˜4.25 (br, 1H), 4.35˜4.65 (m, 3H), 5.00 (2xd, 4H), 7.30 (S, 10H).

B. Dibenzylphosphoryl-L-methionyl-L-prolyl-L-proline L-arginine salt

Dibenzylphosphoryl-L-methionyl-L-prolyl-L-proline methylester (1.87 g, 3.0 m mole) was dissolved in acetone (18 ml), and 1N sodium hydroxide (4.5 ml) was added thereto under cooling and the mixture was stirred for 4 hours. To the solution, water (100 ml) was added, and the solution was washed with diethyl ether. Ethyl acetate (200 ml) was added thereto and the mixture was adjusted to pH 2 with 1N hydrochloric acid while stirring strongly. The ethyl acetate phase was separated and this phase was dried over anhydrous sodium sulfate. The solvent was evaporated to obtain an oily dibenzylphosphoryl-L-methionyl-L-prolyl-L-proline (1.81 g, yield: 99.1%). All of thus obtained product (3.0 m mole) was dissolved in ethanol (15 ml) and L-arginine (0.58 g, 3.0 m mole) water (5 ml) solution was added thereto. The solvent was evaporated and water was added thereto, and thus obtained solution was freeze-dried to obtain dibenzylphosphoryl-L-methionyl-L-prolyl-L-proline L-arginine salt (2.27 g, yield: 98.7% from dibenzylphosphoryl-L-methionyl-L-prolyl-L-proline methylester). This product gave a single spots respectively in R_(f) =0.8 for peptides in the free form and R_(f) =0.25 for arginine by a thin layer chromatography (developing solvent, n-butanol:acetic acid:water=2:1:1, Colour forming method, spraying of 25% hydrobromic acid and 0.1% ninhydrin, and heating). After a hydrolysis of the product with 6N hydrochloric acid, and amino acids were analyzed. Methionine:Proline:Arginine=1.00:2.03:0.95.

EXAMPLE 34

N-Phenethylphosphonyl-L-methionyl-L-prolyl-L-proline 2 sodium salt:

A. N-(O-benzyl-P-phenethylphosphonyl)-L-methionyl-L-prolyl-L-proline benzylester

L-methionyl-L-prolyl-L-proline benzylester hydrochloride (5.69 g, 12.1 m mole) was dissolved in methylene chloride (30 ml), and triethylamine (3.85 g, 38.0 m mole) was added thereto. Methylene chloride solution (20 ml) of monobenzylphenethyl phosphochloridate produced from dibenzylphenethyl phosphonate (6.33 g, 17.3 m mole) was added dropwise to the above solution while cooling in the coolant of -20° C. Further, it was stirred overnight at room temperature. To the reaction solution, methylene chloride (150 ml) was added, and the mixture was washed with 1N hydrochloric acid, water, 5% sodium bicarbonate and water in order, and dried with anhydrous sodium sulfate. The solvent was distilled off under reduced pressure to give a candy-like residue. It was purified by column chromatography (Silica gel, developing solvent; ethyl acetate:methanol=10:1) to give N-(O-benzyl-P-phenethylphosphonyl)-L-methionyl-L-prolyl-L-proline benzylester (2.39 g, yield: 28.5%).

The product gave a single spot with R_(f) =0.7 by thin layer chromatography (developing solvent; ethyl acetate: methanol=10:1, Colour forming method: spraying of 25% hydrobromic acid and 0.1% ninhydrine, and heating). NMR spectrum [CDCl₃, internal standard: TMS] ppm: 1.60˜2.40 (m, 12H), 2.10 (S, 3H), 2.45˜3.20 (m, 4H), 3.25˜3.90 (m, 4H), 3.95˜4.70 (m, 3H), 4.80˜5.30 (m, 5H), 7.18 (S, 5H), 7.32 (S, 10H).

B. N-Phenethylphosphonyl-L-methionyl-L-prolyl-L-proline 2 sodium salt

N-(O-Benzyl-P-phenethylphosphonyl)-methionyl-L-prolyl-L-proline benzylester (1.195 g, 1.73 m mole) was dissolved in ethanol (15 ml), and sodium bicarbonate (0.290 g, 3.46 m mole) in water (5 ml) solution was added thereto. Hydrogen gas was passed through the above solution for 6 hours at room temperature and atmospheric pressure in the presence of 30% palladium barium carbonate as a catalyst. The catalyst was filtered off and from the filtrate the solvent was evaporated. The thus obtained residue was dissolved in water and freeze-dried to obtain N-phenethylphosphonyl-L-methionyl-L-prolyl-L-proline 2 sodium salt (0.80 g, yield: 83.3%). It gave a single spot with R_(f) =0.5 by thin layer chromatography (developing solvent; n-butanol:acetic acid:water=2:1:1, Colour forming method: spraying of 25% hydrobromic acid and 0.1% ninhydrine, and heating).

After hydrolysis of the product with 6N hydrochloric acid, amino acids were analyzed. Methionine:Proline=1.00:2.01.

NMR spectrum [D₂ O, internal standard: DSS] ppm: 1.50˜2.25 (m,12H), 2.12 (S,3H), 2.70 (m, 4H), 3.40˜3.90 (m, 4H), 3.95˜4.40 (m,3H), 7.30 (S, 5H) FAB-MASS spectrum, m/e: 556 (M+1), 578 (M+Na).

EXAMPLE 35

N-(O-Benzyl-P-phenethylphosphonyl)-L-methionyl-L-prolyl-L-proline L-arginine salt:

N-(O-Benzyl-P-phenethylphosphonyl)-L-methionyl-L-prolyl-L-proline benzylester (1.195 g, 1.73 m mole) was dissolved in acetone (10 ml), and 1N sodium hydroxide (2.6 ml) was added thereto under cooling in the ice bath. The mixture was stirred for 5.5 hours. To the solution water (100 ml) was added and the mixture was washed with diethyl ether, and then ethyl acetate (200 ml) was added thereto. The solution was adjusted to pH 2 with 1N hydrochloric acid while stirring vigorously.

Ethyl acetate layer was separated and dried over anhydrous sodium sulfate. The solvent was evaporated to give an oily N-(O-benzyl-P-phenethylphosphonyl)-L-methionyl-L-prolyl-L-proline (1.02 g, yield: 98.1%). All of thus obtained product (1.70 m mole) was dissolved in ethanol (15 ml), and L-arginine (0.33 g, 1.70 m mole) water (5 ml) solution was added thereto. The solvent was distilled off under reduced pressure, and further, water was added thereto. The water solution was freeze-dried to obtain N-(O-benzyl-P-phenethylphosphonyl)-L-methionyl-L-prolyl-L-proline L-arginine salt yielding 94.0% based on N-(O-benzyl-P-phenethylphosphonyl)-L-methionyl-L-prolyl-L-proline benzylester. The product gave single spots respectively in R_(f) =0.7 for peptides in the free form and R_(f) =0.2 for arginine, by thin layer chromatography (developing solvent; n-butanol:acetic acid:water=2:1:1, Color forming method; spraying of 25% hydrobromic acid and 0.1% ninhydrin, and heating).

After hydrolysis of the product with 6N hydrochloric acid, amino acids were analyzed.

Methionine:Proline:Arginine=1.00:2.05:0.89.

EXAMPLE 36

N.sup.α -Phenethylphosphonyl-L-lysyl-L-prolyl-L-proline 2 sodium salt:

A. N.sup.α -(O-Benzyl-P-phenethylphosphonyl)-N.sup.ε -benzyloxycarbonyl-L-lysyl-L-prolyl-L-proline benzylester

N.sup.ε -benzyloxycarbonyl-L-lysyl-L-prolyl-L-proline benzylester hydrochloride (3.26 g, 5.4 m mole) was dissolved in methylene chloride (15 ml), and triethylamine (1.73 g, 17.1 m mole) was added thereto. Methylene chloride (10 ml) solution of monobenzylphenethyl phosphochloridate produced from dibenzylphenethyl phosphonate (2.84 g, 7.8 m mole) was added dropwise and the mixture was stirred overnight at room temperature. To the reaction solution methylene chloride (100 ml) was added, and the mixture was washed with 1N hydrochloric acid, water, 5% sodium bicarbonate and water in order, and dried with anhydrous sodium sulfate. The solvent was distilled off under reduced pressure to obtain a candy-like residue. The residue was purified with column chromatography (Silica gel, developing solvent; ethyl acetate:methanol=20:1) to obtain N.sup.α -(O-benzyl-P-phenethylphosphonyl)-N.sup.ε -benzyloxycarbonyl-L-lysyl-L-prolyl-L-proline benzylester (1.21 g, yield: 27.1%). The product gave a single spot with R_(f) =0.35 by thin layer chromatography (developing solvent; ethyl acetate:methanol=20:1, Colour forming method: spraying of 25% hydrobromic acid and 0.1% ninhydrin, and heating).

B. N.sup.α -Phenethylphosphonyl-L-lysyl-L-prolyl-L-proline 2 sodium salt

N.sup.α -(O-Benzyl-P-phenethylphosphonyl)-N.sup.ε -benzyloxycarbonyl-L-lysyl-L-prolyl-L-proline benzylester (1.21 g, 1.5 m mole) was dissolved in methanol (18 ml), and sodium bicarbonate (0.247 g, 2.9 m mole) water (5 ml) solution was added thereto. Hydrogen gas was passed through the solution for 3 hours at room temperature and atmospheric pressure in the presence of 5% palladium carbon as a catalyst. The catalyst was removed by filtration. The solvent was evaporated and the residue was dissolved in water. The solution was freeze-dried to obtain N.sup.α -phenethylphosphonyl-L-lysyl-L-prolyl-L-proline 2 sodium salt (0.84 g which is yielded quantitatively).

The product gave a single spot with R_(f) =0.3 by thin layer chromatography (developing solvent; n-butanol:acetic acid:water=2:1:1, Colour forming method: spraying of 25% hydrobromic acid and 0.1% ninhydrin, and heating).

After hydrolysis of the product with 6N hydrochloric acid, amino acids were analyzed. Lysine:Proline=1.00:2.02

EXAMPLE 37

N.sup.α -(O-Ethyl-P-phenethylphosphonyl)-L-lysyl-L-prolyl-L-proline L-arginine salt:

A. Diethylphenethylphosphonate

Diethyl phosphite (27.64 g, 200 m mole) was dissolved in DMF (200 ml), and to the solution at nitrogen atmosphere while stirring and -15° C., sodium hydride (60% in oil, 9.6 g, 220 m mole) was added. The mixture was stirred for 1 hour at not more than 0° C. To the solution phenethyl bromide (37.0 g, 200 m mole) was dropwise added and further the mixture was stirred overnight at room temperature. DMF was distilled off under reduced pressure, and the residue was dissolved in ethyl acetate. The ethyl acetate solution was washed with water, and dried with anhydrous sodium sulfate. The solvent was evaporated and the thus obtained oily product was distilled under reduced pressure to obtain diethylphenethylphosphonate (25.0 g, yield: 51.6%) of boiling point 136.5˜137.5° C./3 mmHg.

B. Monoethylphenethyl phosphochloridate

Diethylphenethyl phosphonate (1.73 g, 7.1 m mole) was dissolved in carbon tetrachloride (5 ml) and phosphorus pentachloride (1.55 g, 7.4 m mole) was added to the solution under cooling in the ice bath. The mixture was stirred for 30 minutes. The mixture was heated to 70° C. at the rate of 10° C./15 minutes, and stirred for 30 minutes at 70° C. The solvent and phosphorusoxychloride were distilled off under reduced pressure in the rotary evaporator. Thus produced monoethyl phenethyl phosphochloridate was used without further purification for the following reaction.

C. N.sup.α -(O-Ethyl-P-phenethylphosphonyl)-N.sup.ε -benzyloxycarbonyl-L-lysyl-L-prolyl-L-proline benzylester

N.sup.ε -Benzyloxycarbonyl-L-lysyl-L-prolyl-L-proline benzylester hydrochloride (3.0 g, 5.0 m mole) was dissolved in methylene chloride (15 ml), and triethylamine (1.59 g, 15.7 m mole) was added thereto. Methylene chloride (10 ml) solution of monoethylphenethyl phosphochloridate produced from diethylphenethylphosphonate (1.73 g, 7.1 m mole) was added dropwise to the above solution while cooling in the coolant at -20° C. Further, the mixture was stirred overnight at room temperature. To the reaction solution methylene chloride (100 ml) was added, and the mixture was washed with 1N hydrochloric acid, water, 5% sodium bicarbonate and water in order and dried with anhydrous sodium sulfate. The solvent was distilled off under reduced pressure to obtain a candy-like residue. The residue was purified by column chromatography (Silica gel, developing solvent; ethyl acetate:methanol=20:1) to obtain N.sup.α -(O-ethyl-P-phenethyl phosphonyl)-N.sup.ε -benzyloxycarbonyl-L-lysyl-L-prolyl-L-proline benzylester (2.92 g, yield: 76.9%). The product gave a single spot with R_(f) =0.15 by thin layer chromatography (developing solvent; ethyl acetate:methanol=20:1, Colour forming method: 25% hydrobromic acid and 0.1% ninhydrine, and heating).

D. N.sup.α -(O-Ethyl-P-phenethylphosphonyl)-L-lysyl-L-prolyl-L-proline L-arginine salt

N.sup.α -(O-Ethyl-P-phenethylphosphonyl)-N.sup.ε -benzyloxycarbonyl-L-lysyl-L-prolyl-L-proline benzylester (1.46 g, 1.9 m mole) was dissolved in ethanol (10 ml), and L-arginine (0.40 g, 1.9 m mole) in water (5 ml) solution was added thereto. Hydrogen gas was passed through the solution for 5 hours at atmospheric pressure and room temperature in the presence of 5% palladium carbon as a catalyst. The catalyst was removed by filtration and the solvent was evaporated. Thus obtained residue was dissolved in water and freeze-dried to obtain N.sup.α -(O-ethyl-P-phenethylphosphonyl)-L-lysyl-L-prolyl-L-proline L-arginine salt (1.06 g, yield: 77.7%). The product gave single spots, respectively in R_(f) =0.55 for free peptides and R_(f) =0.2 for arginine by thin layer chromatography (developing solvent; n-butanol:acetic acid:water=2:1:1, Colour forming method: spraying of 25% hydrobromic acid and 0.1% ninhydrine, and heating).

After hydrolysis of the product with 6N hydrochloric acid, amino acids were analyzed.

Lysine:Proline:Arginine=1.00:2.02:0.96.

EXAMPLE 38

N-n-Amylphosphonyl-L-alanyl-L-prolyl-L-proline 2 sodium salt:

A. Monobenzyl n-amylphosphochloridate

Dibenzyl n-amylphosphonate (2.74 g, 8.2 m mole) was dissolved in carbon tetrachloride (5 ml), and phosphorus pentachloride (1.80 g, 8.6 m mole) was added to the solution while cooling in the ice bath, and the mixture was stirred for 30 minutes. The mixture was heated to 70° C. at the rate of 10° C./15 minutes, and further stirred for 30 minutes at 70° C. The solvent, phosphorus oxychloride and benzylchloride were distilled off under reduced pressure in the rotary evaporator. It was confirmed that the starting materials did not remain in the reaction solution by silica gel thin layer chromatography (developing solvent; benzene:ethyl acetate=1:1, Confirmation of spot: UV lamp). Thus produced monobenzyl n-amylphosphochloridate was used without further purification for the following reactions.

B. N-(O-benzyl-P-n-amylphosphonyl)-L-alanyl-L-prolyl-L-proline benzylester

L-Alanyl-L-prolyl-L-proline benzylester hydrochloride (2.37 g, 5.8 m mole) was dissolved in methylene chloride (15 ml), and triethylamine (1.84 g, 18.2 m mole) was added thereto. Methylene chloride (10 ml) solution of monobenzyl-n-amylphosphochloridate produced from dibenzyl n-amylphosphonate (2.74 g, 8.2 m mole) was dropwise added to the above solution while cooling in the coolant at -15° C. The mixture was further stirred overnight at room temperature. To the reaction solution, methylene chloride (100 ml) was added, and the mixture was washed with 1N hydrochloric acid, water, 5% sodium bicarbonate and water and dried over anhydrous sodium sulfate. The solvent was distilled off under reduced pressure to obtain a candy-like residue. It was purified by column chromatography (Silica gel, developing solvent; ethyl acetate:methanol=20:1) to obtain N-(O-benzyl-P-n-amylphosphonyl)-L-alanyl-L-prolyl-L-proline benzylester (1.26 g, yield: 36.5%). The product gave a single spot with R_(f) =0.2 by thin layer chromatography (developing solvent; ethyl acetate:methanol=20:1, Colour forming method: spraying of 25% hydrobromic acid and 0.1% ninhydrine, and heating). NMR spectrum [CDCl₃, internal standard: TMS] ppm: 0.87(t,3H), 1.15˜1.50(m,7H), 1.55˜1.70(m,2H), 1.80˜2.40(m,10H), 3.23˜3.90(m,4H), 3.95˜4.35(m,1H), 4.45˜4.70(m,2H), 4.85˜5.30(m,5H), 7.30(S,10H).

C. N-n-Amylphosphonyl-L-alanyl-L-prolyl-L-proline 2 sodium salt

N-(O-Benzyl-P-n-amylphosphonyl)-L-alanyl-L-prolyl-L-proline benzylester (1.26 g, 2.1 m mole) was dissolved in ethanol (20 ml), and sodium bicarbonate (0.354 g, 4.2 m mole) in water (20 ml) solution was added thereto. Hydrogen gas was passed through the solution at room temperature and atmospheric pressure for 3 hours in the presence of 5% palladium carbon as a catalyst. The catalyst was removed by filtration, and the solvent was evaporated. Thus obtained residue was dissolved in water and freeze-dried to obtain N-n-amylphosphonyl-L-alanyl-L-prolyl-L-proline 2 sodium salt (0.93 g, yield: 95.6%). The product gave a single spot with R_(f) =0.3 by thin layer chromatography (developing solvent; n-butanol:acetic acid:water=2:1:1, Colour forming method: spraying of 25% hydrobromic acid and 0.1% ninhydrine, and heating).

After hydrolysis of the product with 6N hydrochloric acid, amino acids were analyzed. Alanine:Proline=1.00:1.99 NMR spectrum [D₂ O, internal standard: DSS] ppm: 0.86(t,3H), 1.02˜1.70(m,9H), 1.75˜2.50(m,10H), 3.35˜4.35(m,7H).

EXAMPLE 39

Diphenethylphosphinyl-L-alanyl-L-prolyl-L-proline sodium salt:

A. Diphenethyl phosphinic acid

Diethyl phosphite (6.9 g, 50 m mole) was added dropwise to the Grignard reagent solution produced from phenethylbromide (27.5 g, 148 m mole) and magnesium (3.6 g, 148 m mole) in anhydrous diethyl ether (100 ml) while cooling in the ice bath. The mixture was refluxed by heating overnight, and then the remaining Grignard reagent was decomposed by the addition of 1N hydrochloric acid (100 ml) and the diethyl ether was evaporated. Bromine was added to the solution while stirring in the ice bath to give a faintly brown coloured solution. The desired product was extracted with chloroform from the solution. The chloroform layer was dried with anhydrous sodium sulfate, and the chloroform was distilled off under reduced pressure. The thus obtained residue was dissolved in hot ethanol, and the solution was cooled to obtain diphenyl phosphinic acid (10.01 g, yield: 73.0%). NMR spectrum [CDCl₃, internal standard: TMS] ppm: 1.80˜2.23 (m, 4H), 2.70˜3.10 ppm (m, 4H), 7.18 (S, 10H), 11.70 (S, 1H).

B. Diphenethylphosphinylchloride

Diphenethylphosphinic acid (1.67 g, 6.1 m mole) was dissolved in carbon tetrachloride (5 ml), and sulfuryl chloride (1.46 g, 10.8 m mole) was added thereto, and the mixture was heated while refluxing for 2 hours. It was confirmed that the starting material did not remain by tracement of the NMR spectrum. The solvent and excess sulfuryl chloride were distilled off under reduced pressure to obtain an oily diphenethylphosphinylchloride, which was supplied for the following reaction.

C. Diphenethylphosphinyl-L-alanyl-L-prolyl-L-proline benzylester

L-Alanyl-L-prolyl-L-proline benzyl ester hydrochloride (2.0 g, 4.9 m mole) was dissolved in chloroform (20 ml), triethyl amine (1.36 g, 13.4 m mole) was added thereto. Chloroform (8 ml) solution of diphenethyl phosphinyl chloride produced from diphenethylphosphinic acid (1.67 g, 6.1 m mole) was added dropwise to the above solution while cooling in the coolant at -20° C. After the addition, the mixture was stirred for 1 hour at not more than 0° C., and next, overnight at room temperature. To the reaction solution chloroform (100 ml) was added, and the mixture was washed with 1N hydrochloric acid, water, 5% sodium bicarbonate and water in order, and dried over anhydrous sodium sulfate. The solvent was distilled off under reduced pressure to obtain the residue. The residue was re-crystalized with ethyl acetate to obtain diphenethyl phosphinyl-L-alanyl-L-prolyl-L-proline benzylester (1.19 g, yield: 38.7%) of melting point 146˜147° C. The product gave a single spot with R_(f) =0.3 by thin layer chromatography (developing solvent; ethyl acetate:methanol=10:1, Colour forming method: spraying of 25% hydrobromic acid and 0.1% ninhydrine, and heating). NMR spectrum [CDCl₃, internal standard: TMS] ppm: 1.40 (d, 3H), 1.75˜2.35 (m, 12H), 2.65˜3.05 (m, 4H), 3.20˜3.90 (m, 5H), 4.20 (q, 1H) 4.50˜4.75 (m, 2H), 5.10 (q, 2H), 7.20 (S, 10H), 7.32 (S, 5H).

D. Diphenethylphosphinyl-L-alanyl-L-prolyl-L-proline sodium salt

N-Diphenethylphosphinyl-L-alanyl-L-prolyl-L-proline benzylester (0.77 g, 1.2 m mole) was dissolved in methanol (20 ml), and sodium bicarbonate (0.103 g, 1.2 m mole) in water (10 ml) solution was added thereto. Hydrogen gas was passed through the above solution at room temperature and atmospheric pressure for 3 hours in the presence of 5% palladium carbon as a catalyst. The catalyst was removed by filtration, and the solvent was evaporated. The thus obtained residue was dissolved in water and freeze-dried to obtain diphenethyl phosphinyl-L-alanyl-L-prolyl-L-proline sodium salt (0.69 g, which is quantitative). The product gave a single spot with R_(f) =0.65 by thin layer chromatography (developing solvent; n-butanol:acetic acid:water=2:1:1, Colour forming method; spraying of 25% hydrobromic acid and 0.1% ninhydrine, and heating).

After hydrolysis of the product with 6N hydrochloric acid, amino acids were analyzed. Alanine:Proline=1.00:2.01. NMR spectrum [CDCl₃, internal standard: TMS] ppm: 1.40(d,3H), 1.55˜2.35(m,12H), 2.60˜3.00(m,4H), 3.30˜3.80(m,4H), 3.90˜4.30(m,2H), 4.40˜4.70(m,1H), 4.75˜5.20(br,1H), 7.20(S,10H).

EXAMPLE 40˜52

    __________________________________________________________________________                          Example                                                                        No., to                                                                        which                                                     Ex-                  the same                          Amino Acids             am-                  reaction                          Analysis,               ple                  was  Starting         T.L.C.      Treatment of            No.                                                                               Product           applied                                                                             Materials        Rf value    6N                      __________________________________________________________________________                                                            HCl                     40 N--(O--ethyl-P--phenethylphos-                                                                   39   monoethylphenethylphosphochlo-                                                                  n-butanol:acetic                                                                           Met:Pro:Arg =              phonyl)-L-methionyl-L-prolyl-                                                                         ridate; L-methionyl-L-prolyl-                                                                   acid:water = 2:1:1                                                                         1.00:2.05:0.99             L-proline L-arginine salt                                                                             L-proline methylester hydrochlo-                                                                Rf = 0.65 (free peptide),                                     ride             Rf = 0.2 (L-Arginine)               41 N--(O--methyl-P--phenethylphos-                                                                  39   monomethylphenethylphosphochlo-                                                                 n-butanol:acetic                                                                           Met:Pro:Arg =              phonyl)-L-methionyl-L-prolyl-                                                                         ridate, L-methionyl-L-prolyl-L-                                                                 water = 2:1:1 Rf                                                                           1.00:2.01:0.97             L-proline L-arginine salt                                                                             proline methylester hydrochloride                                                               (free peptide), Rf = 0.2                                                       (L-arginine)                        42 N--(O--benzyl-P--phenethylphos-                                                                  26 and                                                                              monobenzylphenethylphosphochlo-                                                                 n-butanol:acetic                                                                           Ala:Pro =                  phonyl)-L-alanyl-L-prolyl-L-                                                                     27   ridate; L-alanyl-L-prolyl-L-                                                                    water= 2:1:1 Rf                                                                            1.00:2.01                  proline sodium salt    proline methylester hydrochlo-                                                 ride                                                 43 N--(O--ethyl-P--phenethylphospho-                                                                39   monoethylphenethylphosphochlo-                                                                  n-butanol:acetic                                                                           Ala:Pro =                  nyl)-L-alanyl-L-prolyl-L-                                                                             ridate; L-alanyl-L-prolyl-L-                                                                    water = 2:1:1Rf                                                                            1.00:2.04                  proline sodium salt    proline benzylester hydrochlo-                                                 ride                                                 44 Diethylphosphoryl-L-methio-                                                                      23   Diethylphosphorylchloride;                                                                      n-butanol:acetic                                                                           Met:Pro:Arg =              nyl-L-prolyl-L-proline L-methionyl-L-prolyl-L-proline                                                                  water = 2:1:1                                                                              1.00:2.02:0.98             L-argine salt          methylester hydrochloride                                                                       Rf = 7.0 (free peptide)                                                        Rf = 0.2 (L-arginine)               45 N--(O--ethyl-P--phenethylphos-                                                                   39   monoethylphenethylphosphochlo-                                                                  ethyl acetate:                                                                             Met:Pro=                   phonyl)-L-alanyl-L-prolyl-                                                                            ridate; L-alanyl-L-prolyl-L-                                                                    methanol:20:1                                                                              1.00:2.05                  L-proline ethylester   proline ethylester hydrochlo-                                                                   Rf = 0.1                                                      ride                                                 46 N--phenethylphosphonyl-L-                                                                        43   monobenzylphenethylphosphochlo-                                                                 n-butanol:acetic                                                                           Ala:Pro =                  alanyl-L-prolyl-L-proline                                                                             ridate; L-alanyl-L-prolyl-L-                                                                    water = 2:1:1                                                                              1.00:1.9                   ethylester sodium salt proline ethyl ester hydrochlo-                                                                  Rf = 0.5                                                      ride                                                 47 N--phenethylphosphonyl-L-alanyl-                                                                 26 and                                                                              monobenzylphenethylphosphochlo-                                                                 n-butanol:acetic                                                                           Ala:Pro:Arg =              L-prolyl-L-proline                                                                               28   ridate; L-alanyl-L-prolyl-L-                                                                    water = 2:1:1                                                                              1.00:2.01:1.98             2 arginine salt        proline benzylester hydrochlo-                                                                  Rf = 0.4 (free peptide)                                       ride             Rf = 0.2 (L-arginine)               48 N--(3-phenylpropyl)phosphonyl-                                                                   26 and                                                                              monobenzyl(3-phenylprophyl)-                                                                    n-butanol:acetic                                                                           Ala:Pro =                  L-alanyl-L-prolyl-L-proline                                                                      28   phosphochloridate; L-alanyl-                                                                    water = 2:1:1                                                                              1.00:2.02                  2 sodium salt          L-prolyl-L-proline benzylester                                                                  Rf =  0.35                                                    hydrochloride                                        49 N--benzylphosphonyl-L-alanyl-                                                                    26 and                                                                              monobenzylbenzylphosphochlori-                                                                  n-butanol:acetic                                                                           Ala:Pro =                  L-prolyl-L-proline 2 sodium                                                                      28   date; L-alanyl-L-prolyl-L-pro-                                                                  water = 2:1:1                                                                              1.00:2.05                  salt                   line benzylester hydrochloride                                                                  Rf = 0.6                            50 N--(O--ethyl-P--phenethylphos-                                                                   39   monoethylphenethylphospho-                                                                      n-butanol:acetic                                                                           Lys:Pro =                  phonyl)-L-lysyl-L-prolyl-                                                                             chloridate; N.sup.6 --benzyloxycar-                                                             water = 2:1:1                                                                              1.00:2.05                  L-proline ethylester   bonyl-L-lysyl-L-prolyl-L-                                                                       Rf = 0.3                               hydrochloride          proline ethylester hydrochlo-                                                  ride                                                 51 N--(O--ethyl-P--phenethyl-                                                                       39   monoethylphenethylphosphochlo-                                                                  n-butanol:acetic                                                                           Lys:Pro =                  phosphonyl)-L-lysyl-L- ridate; N.sup.6 --benzyloxycarbonyl-                                                            water = 2:1:1                                                                              1.00:2.01                  prolyl-L-proline       L-lysyl-L-prolyl-L-proline                                                                      Rf = 0.55                                                     benzylester hydrochloride                            __________________________________________________________________________

EXAMPLE 53

Dibenzylphosphoryl-L-alanyl-L-prolyl-L-proline calcium salt:

Dibenzylphosphoryl-L-alanyl-L-prolyl-L-proline (0.5 g, 0.9 mmol) was dissolved in 1,2-dichloroethane (15 ml) which had been previously saturated with water, and calcium hydroxide (34 mg, 0.465 mmole) was added thereto. The mixture was stirred for 4 hours at room temperature. Insoluble matter was removed by filtration and from the thus obtained filtrate the solvent was distilled off under reduced pressure. The residue was dissolved in ethyl acetate and n-hexane was added thereto while stirring to obtain dibenzylphosphoryl-L-alanyl-L-prolyl-L-proline calcium salt (0.37 g).

Elemental analysis: Found: C 55.92%, H 5.93%, N 7.28% Calculated: C 55.85%, H 6.07%, N 7.23% as C₅₄ H₆₆ N₆ O₁₄ P₂ Ca.2H₂ O.

EXAMPLE 54

Dibenzylphosphoryl-L-alanyl-L-prolyl-L-proline calcium salt:

Dibenzylphosphoryl-L-alanyl-L-prolyl-L-proline (0.5 g, 0.9 mmole) was dissolved in ethyl acetate (15 ml) which had been previously saturated with water, and calcium oxide (50, 0.9 mmol) was added thereto. The mixture was stirred for 2 hours at room temperature. Excess of calcium oxide was removed by filtration, and from the filtrate the solvent was distilled off under reduced pressure. The thus obtained residue was dissovled in ethyl acetate, and n-hexane was added thereto while stirring to obtain dibenzylphosphoryl-L-alanyl-L-prolyl-L-proline calcium salt (0.40 g).

EXAMPLE 55

N-(O-Benzyl-P-phenethylphosphonyl)-L-methionyl-L-prolyl-L-proline calcium salt:

N-(O-Benzyl-P-phenethylphosphonyl)-L-methionyl-L-prolyl-L-proline was reacted with calcium hydroxide in the same manner as in Example 53 to obtain N-(O-Benzyl-P-phenethylphosphonyl)-L-methionyl-L-propyl-L-proline calcium salt.

EXAMPLE 56

Diethylphosphoryl-L-lysyl-L-prolyl-L-proline calcium salt:

Diethylphosphoryl-ε-carbobenzoxy-L-lysyl-L-prolyl-L-proline benzyl ester (2.48 g, 3.5 mmole) was dissolved in methanol (25 ml), and calcium hydroxide (0.133 g) suspended in water (10 ml) was added thereto. The catalytic reduction reaction was carried out in the presence of 5% palladium-carbon as catalyst. The catalyst was removed by filtration and the solvent was distilled off under reduced pressure. The thus obtained milk-white and candy-like residue was dissolved in water. After removal of insoluble matter by filtration, the solution was freeze-dried to obtain diethylphosphoryl-L-lysyl-L-prolyl-L-proline calcium salt (1.54 g, yield: 87.8%).

Mass Spectrum (FAB): m/e 991 (M+1).

EXAMPLE 57

Monobenzylphosphoryl-L-alanyl-L-prolyl-L-proline diammonium salt:

Dibenzylphosphoryl-L-alanyl-L-prolyl-L-proline (1.63 g, 3 mmole) was dissolved in a mixture of ethyl alcohol (50 ml), water (10 ml) and acetic acid (0.1 ml), and 5% palladium-carbon (200 mg) was added thereto. Hydrogen gas was passed through the mixture for 5 minutes while vigorously stirring. To the solution, concentrated ammonia water (1 ml) was added, and then the catalyst was removed by filtration. The filtrate was concentrated to an oily residue. This was dissolved in 1N ammonia water, and the thus obtained solution was passed through a column packed with a resin of porous polystyrene-divinylbenzene, HP-20, produced by Mitsubishi Chemical Industries LTD. The product was eluted from the column with acetonitrile solution, whose concentration was changed linearly from 1N NH₃ water to 1N NH₃ water, containing 33% acetonitrile. The main fractions were collected and the solvent was distilled off to obtain monobenzylphosphoryl-L-alanyl-L-prolyl-L-proline (430 mg, yield: 29.4%).

After hydrolysis of the product with 6N hydrochlorice acid, the amino acids were analyzed. The ratio of alanine, proline an ammonia was 0.92:2.00:1.95.

The product gave a single spot with RF=0.55, by thin layer chromatography with silica gel (developing solvent, n-butanol:acetic acid:water=2:1:1, colour forming method: spraying of 25% hydrobromic acid and 0.1% ninhydrin and heating). Mass Spectrum (FAB): 454 (M+H).

NMR Spectrum (CD₃ OD, Internal Standard: TMS) ppm: 1.25 (d, 3H), 1.60-2.35 (m, 8H), 3.40-3.85 (m, 4H), 3.90-4.45 (m, 3H), 7.33 (S, 5H).

EXAMPLE 58

Monobenzylphosphoryl-L-alanyl-L-prolyl-L-proline di-L-arginine salt:

Dibenzylphosphoryl-L-alanyl-L-prolyl-L-proline di-L-arginine salt:

Dibenzylphosphoryl-L-alanyl-L-prolyl-L-proline (1 g, 1.84 mmole) and L-arginine (0.64 g, 3.67 mmol) were dissolved in a mixture (20 ml) of methanol and water (1:1, v/v) and 5% palladium carbon (0.1 g) was added thereto. Hydrogen gas (41.2 ml, 1.84 mmol) was adsorbed by the solution while stirring. The catalyst was removed by filtration, and the solvent was distilled off under reduced pressure. Water was added thereto and the water solution was freeze-dried to obtain monobenzylphosphoryl-L-alanyl-L-prolyl-L-proline di-L-arginine salt (1.46 g, quantitative yield).

This product gave two spots, one in R_(f) =0.55 for free peptides and another in R_(f) =0.2 for arginine by thin layer chromatography with silica gel (developing solvent, n-butanol:acetic acid:water - 2:1:1, colour forming method: spraying of 25% hydrobromic acid and 0.1% ninhydrine, and heating).

EXAMPLE 59-78

Dibenzylphosphoryl-L-alanyl-L-prolyl-1-prolines in the following salt forms were produced in the same manner as in the above-mentioned Examples. For example, a salt-forming reaction of Example 27 was used for Examples 59-71, 73 and 75, that of Examples 32 for Examples 76 and 77, and that of Example 53 for Example 78.

Dibenzylphosphoryl-L-alanyl-L-prolyl-L-proline ammonium salt (0.92 g) in Example 72 was produced by dissolving dibenzyphiosphoryl-L-alanyl-L-prolyl-L-proline (0.9 g, 1.66 mmol) in 0.83N NH₃ water (2 ml), adding water (100 ml) thereto, and freeze-drying it.

Dibenzylphosphoryl-L-alanyl-L-prolyl-L-proline dicyclohexylamine salt (810 mg, yield: 43.4%) in Example 74 was produced by dissolving dibenzylphosphoryl-L-alanyl-L-prolyl-L-proline (1.4 g, 2.6 mmol) in methanol (5 ml), adding dicyclohexylamine (0.47 g, 2.6 mmole) thereto, removing the solvent by distillation therefrom, dissolving the residue in ethyl acetate (30 ml) while heating, adding to the solution as much diisopropyl ether before production of white muddiness, and cooling it at room temperature.

    ______________________________________                                                             Mass                                                                           Spectrum                                                   Example                                                                               Form of      (FAB)     Amino Acids                                      No.    Salt         (M= H).sup.+                                                                             Analysis                                         ______________________________________                                         59     D-Arginine   718       Alanine:Proline:                                        Salt                   Arginine =                                                                     1.00:1.98:0.97                                   60     DL-Arginine  718       Alanine:Proline:                                        Salt                   Arginine =                                                                     1.00:2.00:1.01                                   61     DL-Lysine    690       Alanine:Proline:                                        Salt                   Lysine =                                                                       1.00:1.95:0.96                                   62     L-Alanine    632       Alanine:Proline =                                       Amide Salt             2.00:1.97                                        63     L-Leucine    674       Alanine:Proline:                                        Amide Salt             Leucine =                                                                      1.00:2.02:0.90                                   64     L-Phenylalanine                                                                             708       Alanine:Proline                                         Amide Salt             Phenylalanine =                                                                1.00:2.05:1.00                                   65     Glycine Amide                                                                               618       Alanine:Proline:                                        Salt                   Glycine =                                                                      1.00:2.07:101                                    66     α -Aminocapro-                                                                        672       Alanine:Proline =                                       lactam Salt            1.00:2.10                                        67     L-Phenylalanine                                                                             723       Alanine:Proline:                                        Methylester Salt       Phenylalanine =                                                                1.00:1.89:1.02                                   68     L-Phenylalanine                                                                             737       Alanine:Proline:                                        Ethylester Salt        Phenylalanine =                                                                1.00:2.00:0.97                                   69     L-Leucine    689       Alanine:Proline:                                        Methylester Salt       Leucine =                                                                      1.00:1.93:0.88                                   70     L-Isoleucine 689       Alanine:Proline:                                        Methylester Salt       Isoleucine =                                                                   1.00:1.90:0.94                                   71     L-Valine     675       Alanine:Proline:                                        Methylester Salt       Valine =                                                                       1.00:1.92:0.99                                   72     Ammonium Salt                                                                               561       Alanine:Proline =                                                              1.00:2.02                                        73     t-Butyl Amine                                                                               617       Alanine:Proline                                         Salt                   1.00:1.94                                        74     Dicyclohexyl 725       Alanine:Proline =                                       Amine Salt             1.00:2.00                                        75     N--Methyl-D- 739       Alanine:Proline =                                       Glucamine Salt         1.00:1.98                                        76     Lithium Salt 550       Alanine:Proline =                                                              1.00:2.06                                        77     Sodium Salt  566       Alanine:Proline =                                                              1.00:1.89                                        78     Magnesium Salt                                                                              1109      Alanine:Proline =                                                              1.00:2.00                                        ______________________________________                                    

EXAMPLE 79

The antihypertensive activity of part of the amino acid derivatives prepared above, was determined. As subject animals 2 SHR were used which had been trained enough and been confirmed to be hypertensive spontaneously hypertensive rats (male, 13 months of age, the body weight of 400 to 440 g) per sample. As an instrument for measuring the blood pressure a Programmed Electro-Sphygmomanometer PE-300 (Narco Co., U.S.A.) was used, and the blood pressure was indirectly measured on conscious rats.

An aqueous solution or suspension of a sample (35 mg/kg) was once force-fed into the stomach by means of a peroral probe. Table shows the results.

                  TABLE 2                                                          ______________________________________                                         Systolic Blood Pressure, mmHg                                                                         4 hours after                                                                             Δ Blood                                Sample    Pretreatment administration                                                                            Pressure                                     ______________________________________                                         Example 53                                                                               220          188        -32                                          Example 55                                                                               210          180        -30                                          Example 56                                                                               204          181        -23                                          Example 57                                                                               195          167        -28                                          Example 74                                                                               195          165        -30                                          ______________________________________                                    

EXAMPLE 80

N-Phenethylphosphonyl-L-alanyl-L-prolyl-L-proline methylester sodium salt:

A. N-(O-Benzyl-P-phenethylphosphonyl)-L-alanyl-L-prolyl-L-proline methylester L-Alanyl-L-prolyl-L-proline methylester hydrochloride (3.3 g, 10.0 mmole) was dissolved in methylene chloride (30 ml), and triethylamine (3.03 g, 30.0 mmole) was added thereto.

Methylene chloride (20 ml) solution of monobenzylphenethyl-phosphochloridate produced from dibenzylphenethylphosphonate (5.23 g, 14.3 mmole) was added dropwise to the above solution while cooling in the ice bath. The mixture was stirred overnight at room temperature. To the reaction solution methylene chloride (150 ml) was added, and the mixture was washed with lN hydrochloric acid, water, 5% sodium bicarbonate and water in order, and dried with anhydrous sodium sulfate. The solvent was distilled off under reduced pressure to obtain a candy-like residue. It was purified by column chromatography (Silica gel, developing solvent; ethyl acetate:methanol=10:1) to give N-(O-benzyl-P-phenethylphosphonyl)-L-alanyl-L-prolyl-L-proline methylester (1.91 g, yield: 34.4%). The product gave a single spot with Rf=0.2 by thin layer chromatography (developing solvent; ethylacetate:methanol=2:1, Colour forming method: spraying of 25% hydrobromic acid and 0.1% ninhydrine, and heating).

NMR spectrum [CDCl₃, internal standard: TMS] ppm: 1.33(2 x d, 3H); 1.75˜2.40(m,10H), 2.60˜3.15(m,2H), 3.25˜3.90(m,4H), 3.68(S,3H), 4.00˜4.35(br,lH), 4.50˜4.75(m,3H), 4.98(2 x d, 2H), 7.20(S,5H), 7.35(S,5H).

B. N-Phenethylphosphonyl-L-alanyl-L-prolyl-L-proline methylester sodium salt

N-(O-Benzyl-P-phenethylphosphonyl)-L-alanyl-L-prolyl-L-proline methylester (1.21 g,2.2 mmole) was dissolved in ethanol (20 ml), and sodium bicarbonate (0.18 g,2.2 mmole) in water (5 ml) solution was added thereto. Hydrogen gas was passed through the above solution for 90 minutes at room temperature and atmospheric pressure in the presence of 5% palladium carbon as a catalyst. The catalyst was removed by filtration, and the solvent was distilled off. The thus obtained residue was dissolved in water, and the solution was freeze-dried to obtain N-phenethyl-phosphonyl-L-alanyl-L-prolyl-L-proline methylester sodium salt (0.95 g, yield: 89.5%). The product gave a single spot with Rf=0.45 by thin layer chromatography (developing solvent; n-butanol:acetic acid:water=2:1:1, Colour forming method: spraying of 25% hydrobromic acid and 0.1% ninhydrine, and heating). NMR spectum [CDCl₃, internal standard: TMS] ppm: 1.20(d,3H), 1.50˜2.35(m,10H), 2.55˜3.05(m,2H), 3.20˜4.10 (m,5H), 3.65(S,3H), 4.25˜4.90(m,3H), 7.10(s,5H).

EXAMPLE 81

An antihypertensive activity of part of the amino acid derivatives prepared above was determined.

As subject animals there were used 2 SHR which had been trained enough and been confirmed to be hypertensive spontaneously hypertensive rats (male, 13 months of age, the body weight of 400˜440 g) per sample.

As an instrument for measuring the blood pressure there was used Programmed Electro-Sphygmomanometer PE-300 (Narco Co., U.S.A.), and the blood pressure was indirectly measured on conscious rats.

An aqueous solution or suspension of a sample (35 mg/kg) was once force-fed into the stomach by means of a peroral probe. Table 1 shows the results.

                  TABLE 1                                                          ______________________________________                                         Systolic Blood Pressure, mmHg                                                                      4 hrs after                                                Sample  Pretreatment                                                                               administration                                                                            Δ Blood Pressure                          ______________________________________                                         Example 1                                                                              205         170        -35                                             Example 2                                                                              202         180        -22                                             Example 3                                                                              208         197        -11                                             Example 4                                                                              207         195        -12                                             Example 5                                                                              188         173        -15                                             Example 6                                                                              212         190        -22                                             Example 8                                                                              196         177        -19                                             Example 12                                                                             193         182        -11                                             Example 13                                                                             181         162        -19                                             Example 14                                                                             183         161        -22                                             Example 15                                                                             182         167        -15                                             Example 16                                                                             181         162        -19                                             Example 17                                                                             182         163        -19                                             Example 18                                                                             192         171        -21                                             Example 19                                                                             196         181        -15                                             Example 20                                                                             184         169        -15                                             Example 21                                                                             191         167        -24                                             Example 22                                                                             206         196        -10                                             Example 23                                                                             190         165        -25                                             Example 24                                                                             192         161        -31                                             Example 25                                                                             183         156        -27                                             Example 26                                                                             182         160        -22                                             Example 27                                                                             180         152        -28                                             Example 28                                                                             183         151        -32                                             Example 29                                                                             203         172        -31                                             Example 30                                                                             184         154        -30                                             Example 31                                                                             199         169        -30                                             Example 32                                                                             185         160        -25                                             Example 33                                                                             186         168        -18                                             Example 34                                                                             183         154        -29                                             Example 35                                                                             191         158        -33                                             Example 36                                                                             200         177        -23                                             Example 37                                                                             201         172        -29                                             Example 38                                                                             187         167        -20                                             Example 39                                                                             195         172        -23                                             Example 40                                                                             196         166        -30                                             Example 41                                                                             203         172        -31                                             Example 42                                                                             194         180        -14                                             Example 43                                                                             195         171        -24                                             Example 44                                                                             189         179        -10                                             Example 80                                                                             193         183        -10                                             *       198         193        -5                                              ______________________________________                                          *Dibenzyl phosphorylL-alanyl-L-proline Larginine salt                    

EXAMPLE 82

Dry packed capsules containing 50 mg/capsule of an active ingredient were prepared.

Dibenzylphosphoryl-L-alanyl-L-prolyl-L-proline L-arginate: 50 mg

Lactose: 149 mg

Magnesium stearate: 1 mg

Capsule (size No. 1): 200 mg

An amino acid derivative was reduced to a No. 60 powder. Lactose and magnesium stearate were passed through a No. 60 sieve cloth to fall over the foregoing powder and mixed sufficiently with it. The mixture was packed into No. 1 dry gelatin capsules.

EXAMPLE 83

There were prepared tablets containing dibenzylphosphoryl-L-alanyl-L-prolyl-L-proline L-arginate (25 mg), pregelatinized starch (82 mg), microcrystalline cellulose (82 mg), and magnesium stearate (1 mg). In place of the foregoing amino acid derivative, other amino acid derivatives described hereinbefore were able to be made into tablets together with pregelatinized starch, microcrystalline cellulose, and magnesium stearate.

Tablets for combined use containing a diuretic such as hydrochlorothiazide were prepared by incorporating therein dibenzylphosphoryl-L-alanyl-L-prolyl-L-proline-L-arginate (7.5 mg), hydrochlorothiazide (50 mg), pregelatinized starch (82 mg), microcrystalline cellulose (82 mg), and magnesium stearate (1 mg).

The foregoing results demonstrate that the amino acid derivatives of the present invention are useful as an antihypertensive drug or an intermediate for preparation thereof. 

What is claimed is:
 1. An amino acid derivative represented by the formula: ##STR7## wherein: R₁ and R₂ are the same or different from each other, and each individually represents hydroxy, alkyloxy, aryloxy, aralkyoxy, alkyl, aryl, or aralkyl;R₃, R₅, R₈, and R₁₁, at least two of which are the same, or all of which are different from one another, and each individually represents a hydrogen atom or lower alkyl; R₄, R₇, and R₁₀, at least two of which are the same, or all of which are different from one another, and each individually represents a hydrogen atom, lower alkyl, lower aryl, or benzyl which may be further substituted by OH, COOH, amino, carbamoyl, guanidino, imidazolyl, indolyl, mercapto, or alkylthio; R₆ and R₉ are the same or different from each other, and each individually represents hydrogen atom, lower alkyl, lower aryl, or benzyl; R₁₂ represents hyroxyl, alkyloxy, aryloxy, aralkyloxy, amino, mono- or di-alkyl-, aryl-, or aralkylamino; and R₆ and R₇ combined together, and R₉ and R₁₀ combined together, may independently form an alkylene bridge having 2 to 4 carbon atoms, which may further contain a double bond, or be further substituted by hydroxyl.
 2. The derivative as described in claim 1, wherein at least one of amino acids constituting said amino acid derivative is in the L-form.
 3. The derivative as described in claim 1, wherein said amino acid derivative is in the salt form.
 4. The derivative as described in claim 1, wherein R₃, R₅, R₈ and R₁₁ are hydrogen atoms.
 5. The derivative described in claim 1, wherein:R₁ and R₂ are the same or different from each other, and each individually represents hydroxyl, a lower alkyloxy having 1 to 5 carbon atoms, an aryloxy having 6 to 12 carbon atoms, an aralkyloxy having 6 to 12 carbon atoms, a lower alkyl having 1 to 5 carbon atoms, an aryl having 6 to 12 carbon atoms, or an aralkyl having 6 to 12 carbon atoms; R₃, R₅, R₈, and R₁₁, at least two of which are the same, or all of which are different from one another, and each individually represents hydrogen atom or a lower alkyl having 1 to 5 carbon atoms; R₄, R₇, and R₁₀, at least two of which are the same, or all of which are different from one another, and each individually represents a hydrogen atom, or lower alkyl having 1 to 5 carbon atoms, lower aryl having 1 to 5 C atoms, or benzyl, which may be further substituted by HO, COOH, amino, carbamoyl, guinidino, imidazolyl, indolyl, mercapto or alkylthio; R₆ and R₉ are the same or different from each other, and each individually represents a hydrogen atom, a lower alkyl having 1 to 5 carbon atoms, an allyl having 6 to 12 carbon atoms or benzyl; and R₁₂ represents hydroxyl, a lower alkyl having 1 to 5 carbon atoms, an aryloxy having 6 to 12 carbon atoms, aralkyloxy having 6 to 12 carbon atoms, an amino, mono-or di-lower alkyl amino having 1 to 5 carbon atoms, aryl amino having 6 to 12 carbon atoms or aralkyl amino having 6 to 12 carbon atoms.
 6. The derivative as described in claim 1, wherein when an alkyl, an aryl or an aralkyl represented as R₄, R₇ and R₁₀ has at least one substitute group, the substitute group is an organic group selected from the group consisting of hydroxyl, carboxyl, carbamoyl, amino, guanidino, imidazolyl, Indolyl, mercapto and lower alkylthio.
 7. The derivative as described in claim 1, wherein when R₆ and R₇, combined together, or R₉ and R₁₀ combined together, and form an alkylene bridge having at least one substitute group, the substitute group is an organic group selected from the group consisting of hydroxyl, lower alkoxy, lower alkyl, oxo, condensed aryl ring and a condensed alicyclic ring.
 8. The derivative as described in claim 1, wherein R₁ and R₂ are the same or different from each other and individually represents an organic group selected from the group consisting of hydroxyl, methoxy, ethoxy, n-propyloxy, n-butyloxy, phenoxy, benzyloxy, phenethyloxy, methyl, ethyl, n-propyl, n-butyl, n-pentyl, phenyl, benzyl, phenethyl, and phenylpropyl, R₃, R₅, R₈ and R₁₁ represent hydrogen atom, or at least one of them represents methyl instead of the hydrogen atom,R₄, R₇ and R₁₀ at least two of which are the same or all of which are different from one another, each individually represents an organic group selected from the group consisting of hydrogen atom, methyl, isobutyl, phenyl, benzyl, hydroxy-benzyl, hydroxymethyl, carboxymethyl, carboxyethyl, carbamoyl-methyl, carbamoylethyl, aminopropyl, aminobutyl, aminopentyl, guanidinopropyl, imidazolylmethyl, Indolylmethyl, mercaptomethyl and methylthioethyl, R₆ and R₉ are the same or different from each other and each individually represents an organic group selected from the group consisting of hydrogen atom, methyl, cyclopropyl, cyclopentyl, cyclohexyl, 2-indanyl and 1-indanyl, and R₁₂ represents an organic group selected from the group consisting of hydroxyl, methyloxy, ethyloxy, benzyloxy, amino, methylamino, ethylamino and phenyloxy.
 9. The derivative as described in claim 1, wherein when R₆ and R₇ are combined together, or R₉ and R₁₀ are combined together, the structure of the combination being the following: ##STR8##
 10. The derivative as described in claim 3, wherein said salt is one selected from the group consisting of sodium salt, potassium salt, lithium salt, ammonium salt, dicyclohexylamine salt, t-butylamine salt and basic amino acid salt.
 11. An antihypertensive composition which comprises0.2-2,000 mg of at least one of the amino acid derivatives represented by the formula: ##STR9## wherein: R₁ and R₂ are the same or different from each other, and each individually represents hydroxyl, alkyloxy, aryloxy, aralkyloxy, alkyl, aryl, or aralkyl; R₃, R₅, R₈, and R₁₁, at least two of which are the same, or all of which are different from one another, and each individually represents hydrogen atom or lower alkyl;R₄, R₇, and R₁₀, at least two of which are the same, or all of which are different from one another, and each individually represents a hydrogen atom, or lower alkyl, lower aryl or benzyl, which may be further substituted by OH, COOH, amino, carbamoyl, guanidino, imidazolyl, indolyl, mercapto, or alkylthio; R₆ and R₉ are the same or different from each other, and each individually represents a hydrogen atom, lower alkyl, lower aryl or benzyl; R₁₂ represents hydroxyl, alkyloxy, aryloxy, aralkyloxy, amino, mono- or di-alkyl, aryl, or aralkylamino; and R₆ and R₇ combined together, and R₉ and R₁₀ combined together, may independently form an alkylene bridge having 2 to 4 carbon atoms, which may further contain a double bond; and a pharmaceutically acceptable carrier.
 12. The antihypertensivce composition as described in claim 11, wherein at least one of amino acids constituting said amino acid derivative is in the L-form.
 13. The antihypertensive composition as described in claim 11, wherein said amino acid derivative is in the form of the pharmaceutically acceptable salt.
 14. The antihypertensive composition as described in claim 11, which further contains a diuretic. 